LOCUS       sequence-140176-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 (S956 to end domain) in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Plekhm1 pFastHFTb
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Plekhm1 -C101 pGEX 4T2-GST
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..22
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(394..416)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(561..579)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        647..751
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatctggtt ccgcgtggat cctctccgca taggttcagt gttgctgacc tccaacagat
       61 cgcagacggg gtgtatgaag gattcctcaa ggccctgatt gaatttgcct cccagcatgt
      121 ctaccactgc gacctgtgca cccagcgcgg cttcatctgc cagatctgcc agcaccacga
      181 catcatcttc ccctttgagt ttgacaccac agtcaggtgt gccgagtgca agaccgtctt
      241 ccaccagagc tgccaggctg tggtgaagaa gggctgcccc cgctgtgccc gccggcgcaa
      301 gtaccaggaa cagaacattt tcgcctgact cgagcggccg catcgtgact gactgacgat
      361 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
      421 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      481 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
      541 taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      601 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      661 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      721 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc cgtgtcgccc
      781 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
//
