LOCUS       sequence-140178-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 (S956 to end domain) in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  TITLE     Plekhm1 pFastHFTb
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Plekhm1 -C101 pGEX 4T2-GST
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(350..367)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(394..416)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gcgcgaggca gatcgtcagt cagtcacgat gcggccgctc gagtcaggcg aaaatgttct
       61 gttcctggta cttgcgccgg cgggcacagc gggggcagcc cttcttcacc acagcctggc
      121 agctctggtg gaagacggtc ttgcactcgg cacacctgac tgtggtgtca aactcaaagg
      181 ggaagatgat gtcgtggtgc tggcagatct ggcagatgaa gccgcgctgg gtgcacaggt
      241 cgcagtggta gacatgctgg gaggcaaatt caatcagggc cttgaggaat ccttcataca
      301 ccccgtctgc gatctgttgg aggtcagcaa cactgaacct atgcggagag gatccacgcg
      361 gaaccagatc cgattttgga ggatggtcgc caccaccaaa cgtggcttgc cagccctgca
      421 aaggccatgc tatatacttg ctggatttca agtacttatc aatttgtggg atagcttcaa
      481 tacgtttttt aaaacaaact aattttggga acgcatccag gcacattggg tccatgtata
      541 aaacaacatc aagagcgtca tacaacatga agtcaggatg ggttacatga tcaccattta
      601 aatatgtttt atgacataaa cgatcttcga acattttcag catttcaggt agcttgctaa
      661 gaaaatcaac tttgagagtt tcaaagtctt tactatatgc aattctcgaa acaccgtatc
      721 taatatccaa aaccgctcct tcaagcattg aaatctctgc acgctctttt ggacaaccac
      781 ccaacatgtt gtgcttgtca gctatataac gtatgatggc catagactgt gttaatttaa
      841 catcaccatc aatataataa ggaagattgg gaaactccaa acccaattca aactttttgt
      901 ttcgccattt atcaccttca tcgcgctcat acaaatgctc ttcatatttt tcttcaagat
      961 attccaaaag aagtcgagtg ggttgcacaa
//
