LOCUS       sequence-140180-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 (PH1 domain) in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     Plekhm1 pFastHFTb
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Plekhm1- PH1 pGEX 4T2-GST
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(465..487)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(632..650)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        718..822
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctggttcgc gtggatccca ggggcataag agcttccggg tggtacaccg gagacagatg
       61 ggactgtcca acccattccg gggtctcatg aagctgggca ccgtggagcg gcggggggca
      121 atgggcatct ggaaggagct cttctgcgag ctctccccgc tggagttccg cctctacctg
      181 agcaacgagg agcacacctg tgtggagaac tgctcgctgc ttcgctgtga gtctgtgggg
      241 ccagcccata gtgatgggcg ctttgagctg gtcttctctg gcaagaagct ggccctgcgc
      301 gcctcctccc aggacgaagc tgaggactgg ctggaccggg tgcgggaggc cctgcagaag
      361 gtccggcctc agcaggagga tgagtgggtg aacgtgtgac tcgagcggcc gcatcgtgac
      421 tgactgacga tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
      481 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
      541 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
      601 agcggagtgt ataattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
      661 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      721 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      781 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      841 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacca
//
