LOCUS       sequence-140181-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 (PH1 domain) in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  TITLE     Plekhm1 pFastHFTb
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Plekhm1- PH1 pGEX 4T2-GST
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(420..437)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(464..486)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 aggcagatcg tcagtcagtc acgatgcggc cgctcgagtc acacgttcac ccactcatcc
       61 tcctgctgag gccggacctt ctgcagggcc tcccgcaccc ggtccagcca gtcctcagct
      121 tcgtcctggg aggaggcgcg cagggccagc ttcttgccag agaagaccag ctcaaagcgc
      181 ccatcactat gggctggccc cacagactca cagcgaagca gcgagcagtt ctccacacag
      241 gtgtgctcct cgttgctcag gtagaggcgg aactccagcg gggagagctc gcagaagagc
      301 tccttccaga tgcccattgc cccccgccgc tccacggtgc ccagcttcat gagaccccgg
      361 aatgggttgg acagtcccat ctgtctccgg tgtaccaccc ggaagctctt atgcccctgg
      421 gatccacgcg gaaccagatc cgattttgga ggatggtcgc caccaccaaa cgtggcttgc
      481 cagccctgca aaggccatgc tatatacttg ctggatttca agtacttatc aatttgtggg
      541 atagcttcaa tacgtttttt aaaacaaact aattttggga acgcatccag gcacattggg
      601 tccatgtata aaacaacatc aagagcgtca tacaacatga agtcaggatg ggttacatga
      661 tcaccattta aatatgtttt atgacataaa cgatcttcga acattttcag catttcaggt
      721 agcttgctaa gaaaatcaac tttgagagtt tcaaagtctt tactatatgc aattctcgaa
      781 acaccgtatc taatatccaa aaccgctcct tca
//
