LOCUS       sequence-140186-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 (PH2 domain) in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  TITLE     Plekhm1 pFastHFTb
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Plekhm1- PH2 pGEX 4T2-GST
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(482..499)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(526..548)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cgaggcagat cgtcagtcag tcacgatgcg gccgctcgag tcactcggct gtctccaagt
       61 aggatgccag gactttgcgg accagatccc tccacagggc ggcttcctcg gcgtttccgg
      121 cctgcagctt caggacagcc ttggccgtga tgattttgaa gaaggatggg cccccaaggc
      181 tggtgtctgg caggatgtcc cggatggtct ccacgccgtg gctgtcactc agcatcttct
      241 cattgttcct gatgcggaaa catttcagag cctccaagga cagagaaaat atatagggca
      301 tccaggtcct gtccatgtac aagtacagca gggactcctt gatggcatct ggctctggaa
      361 cctgggcgga cgaccagtca aactgtgtgc cctggagggc cgcgggctcc gagagcaggt
      421 ctgagggaga gaggcagccc tggggcgcct cggggggttc ctcaggctgg tctgggtact
      481 gggatccacg cggaaccaga tccgattttg gaggatggtc gccaccacca aacgtggctt
      541 gccagccctg caaaggccat gctatatact tgctggattt caagtactta tcaatttgtg
      601 ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc aggcacattg
      661 ggtccatgta taaaacaaca tcaagagcgt catacaacat gaagtcagga tgggttacat
      721 gatcaccatt taaatatgtt ttatgacata aacgatcttc gaacattttc agcatttcag
      781 gtagcttgct aagaaaatca actttgagag tttcaaagtc tttactatat gcaattctcg
      841 aaacaccgta tctaatatcc aaaaccgctc cttcaagcat tgaaatctct gcacgctctt
      901 ttggaca
//
