LOCUS       sequence-140212-         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Zhang P, Medwig-Kinney TN, Goldstein B
  TITLE     Architecture of the cortical actomyosin network driving apical 
            constriction in C. elegans.
  JOURNAL   J Cell Biol. 2023 Sep 4;222(9):e202302102. doi: 
            10.1083/jcb.202302102. Epub 2023 Jun 23.
  PUBMED    37351566
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-09-4"; volume: "222(9):e202302102. doi"; pages: 
            " 10.1083/jcb.202302102. Epub 2023 Jun 23"
COMMENT     Sequence Label: pDD315 (mTurquoise2^SEC^2xHA)
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(566..585)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttgacctcgg cacgggtctt gtagtttccg tcgtccttga agaagatggt acgctcttgg
       61 acgtatccct ctggcatggc ggacttgaag aagtcgtgtt gcttcatgtg gtctgaaaaa
      121 atcctccaac ttcattacat tgaatataca ttcccaaact tacctgggta acgggcgaag
      181 cattggactc cccaggagag ggtggtgacg agggttggcc atgggactgg gagctttccg
      241 gtggtgcaga tgaacttgag ggtgagcttt ccgtaggtgg cgtctccctc tccctctccg
      301 gagacggaga acttgtgtcc gttgacgtct ccgtcgagct cgacgaggat tggtactacc
      361 cccgtaaaca attcttcgcc cttacttacc atcgatgctc ctgaggctcc cgatgctccc
      421 taggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
      481 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
      541 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
      601 cgatatgcac caccgggtaa agttcacggg ggactttatc tgacagcaga cgtgcactgg
      661 ccaggg
//
