LOCUS       sequence-140216-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from 
            Bba_B0030 to adjust spacing in MC system. [B:B0030m:C].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Densmore
  TITLE     B0030m_BC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: B0030m_BC
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..64)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    67..88
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             114..125
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    142..162
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(168..185)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(419..438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
       61 tttctggaat tcgcggccgc ttctagagac tagtgggtct catactagag attaaagagg
      121 agaaatacta aatgagagac ctactagtag cggccgctgc aggcttcctc gctcactgac
      181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
      361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      841 tcttgatccg gcaaacaaac caccgctggt agcggtgg
//
