LOCUS       sequence-140249-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6349 MSCV-CMV-Flag-HA-Brd4 1224-1362
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..25
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     56..80
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             162..185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             198..224
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    240..264
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             288..308
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(745..769)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(823..846)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
       61 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      121 gacaccggga ccgatccagc ctccggggat ctgccaccat ggactacaag gatgacgatg
      181 acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga ggttatcaaa
      241 caagtttgta caaaaaagca ggctccgcgg ccgccccctt cacccctcct aagaagaaga
      301 ggaaggtgtt cgagcagttc cgccgcgccg ctcgggagaa agaggagcgt gagaaggccc
      361 tgaaggctca ggccgagcac gctgagaagg agaaggagcg gctgcggcag gagcgcatga
      421 ggagccgaga ggacgaggat gcgctggagc aggcccggcg ggcccatgag gaggcacgtc
      481 ggcgccagga gcagcagcag cagcagcgcc aggagcaaca gcagcagcag caacagcaag
      541 cagctgcggt ggctgccgcc gccaccccac aggcccagag ctcccagccc cagtccatgc
      601 tggaccagca gagggagttg gcccggaagc gggagcagga gcgaagacgc cgggaagcca
      661 tggcagctac cattgacatg aatttccaga gtgatctatt gtcaatattt gaagaaaatc
      721 ttttctgaaa gggtgggcgc gccgacccag ctttcttgta caaagtggtt cgataacgaa
      781 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
      841 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
      901 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
      961 ctagtcagga agttcccccc cgcccc
//
