LOCUS       sequence-140252-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6345 MSCV-CMV-Flag-HA-Brd4 1-722
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..22
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     53..77
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      150..159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             159..182
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             195..221
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    237..261
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             282..302
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt
       61 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      121 accgggaccg atccagcctc cggggatctg ccaccatgga ctacaaggat gacgatgaca
      181 agctcgatgg aggataccca tacgatgttc cagattacgc tgctcgaggt tatcaaacaa
      241 gtttgtacaa aaaagcaggc tccgcggccg ccgtactcga ggaaaacctg tattttcagg
      301 gctccttcac ctctgcggag agcggccctg ggacgagatt gagaaatctg ccagtaatgg
      361 gggatggact agaaacttcc caaatgtcta caacacaggc ccaggcccaa ccccagccag
      421 ccaacgcagc cagcaccaac cccccgcccc cagagacctc caaccctaac aagcccaaga
      481 ggcagaccaa ccaactgcaa tacctgctca gagtggtgct caagacacta tggaaacacc
      541 agtttgcatg gcctttccag cagcctgtgg atgccgtcaa gctgaacctc cctgattact
      601 ataagatcat taaaacgcct atggatatgg gaacaataaa gaagcgcttg gaaaacaact
      661 attactggaa tgctcaggaa tgtatccagg acttcaacac tatgtttaca aattgttaca
      721 tctacaacaa gcctggagat gacatagtct taatggcaga agctctggaa aagctcttct
      781 tgcaaaaaat aaatgagcta cccacagaag aaaccgagat catgatagtc caggcaaaag
      841 gaagaggacg tgggaggaaa gaaacaggga cagcaaaacc tggcgtttcc acggtaccaa
      901 acacaactca ag
//
