LOCUS       sequence-140260-        6461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     Sequence Label: pMXs-gw
FEATURES             Location/Qualifiers
     source          1..6461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(100..204)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             234..827
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    890..1247
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1306..1722
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1652..1674
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1690..1706
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1732..2106
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2044..2060
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    2157..2281
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2306..2311,2312..2329,2330..2336)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2390..3049
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2456..2475)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3391..3696
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3575..3594
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3737..3861)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             4178..4647
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4777..4794)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4948..5536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5028..5047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5700..6461,1..30,31..99))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6323..6342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
       61 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
      121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
      181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cataatgaaa
      241 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
      301 aatacataac tgagaataga aaagttcaga tcaaggtcag gaacagatgg aacagctgaa
      361 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
      421 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
      481 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
      541 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
      601 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
      661 agcccacaac ccctcactcg gcgcgccagt cctccgattg actgagtcgc ccgggtaccc
      721 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
      781 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
      841 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag
      901 caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc
      961 ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg
     1021 gaacacccgg ccgcaaccct gggagacgtc ccagggactt cgggggccgt ttttgtggcc
     1081 cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg gtgcaccccc ctaataggag
     1141 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt
     1201 ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg
     1261 tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc
     1321 actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag
     1381 tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt
     1441 aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc
     1501 aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg
     1561 gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg
     1621 cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct
     1681 cgatcctccc tttatccagc cctcactcct tctctaggcg cccccatatg gccatatgag
     1741 atcttatatg gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt
     1801 actaacagcc cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc
     1861 tggagacctc tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct
     1921 taccgagtcg gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc
     1981 tggaaaggac cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc
     2041 gcagcttgga tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct
     2101 agactgccgg atctagctag ttaattaagg atcccagtgt ggtggtacgg gaaatcacaa
     2161 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     2221 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     2281 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     2341 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     2401 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     2461 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     2521 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     2581 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     2641 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     2701 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     2761 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     2821 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     2881 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     2941 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     3001 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc
     3061 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
     3121 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt
     3181 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
     3241 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
     3301 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
     3361 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga
     3421 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
     3481 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     3541 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     3601 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     3661 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
     3721 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     3781 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     3841 agctttcttg tacaaagtgg tgatattcca gctgagcgcc ggtcgctacc attaccagtt
     3901 ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat ttcgcgtaag
     3961 gaaatccatt atgtactatt taaactcgag cggccgccag cacagtggtc gacgataaaa
     4021 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     4081 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
     4141 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
     4201 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
     4261 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     4321 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     4381 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     4441 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     4501 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     4561 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     4621 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt
     4681 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg
     4741 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4801 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4861 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4921 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5221 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5281 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5341 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5401 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5461 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5521 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5581 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5641 ccttttgcgg ccggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5701 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5761 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5821 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     5881 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5941 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6001 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6061 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6121 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6181 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6241 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6301 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6361 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6421 agttcgatgt aacccactcg tgcacccaac tgatcttcag c
//
