LOCUS       sequence-140275-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nabi2.30 is a FRET (Forster Resonance Energy Transfer)-based protein
            voltage sensor. This probe contains Clover and mRuby2 inserted at 
            different locations in the Ciona voltage sensitive domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Sung U, Sepehri-Rad M, Piao HH, Jin L, Hughes T, Cohen LB, Baker BJ
  TITLE     Developing Fast Fluorescent Protein Voltage Sensors by Optimizing 
            FRET Interactions.
  JOURNAL   PLoS One. 2015 Nov 20;10(11):e0141585. doi: 
            10.1371/journal.pone.0141585. eCollection 2015.
  PUBMED    26587834
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-11-20"; volume: "10(11):e0141585. doi"; pages: " 
            10.1371/journal.pone.0141585. eCollection 2015"
COMMENT     Sequence Label: Nabi2.30
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    112..336
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     325..342
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
       61 gcgcgtgggg atacccccta gagccccagc tggttctttc cgcctcagaa gccatagagc
      121 ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc
      181 ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat
      241 tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg
      301 ggcaaacaac agatggctgg caactagaag gcacagtcga gggcgcgcct attacttgta
      361 cagctcgtcc atcccaccac caagcccggc gaacttggca actgcgtgtt cgcgttgtac
      421 tacgaacatt tcattgtcac tttcctctaa cctttccagg cggtgatcaa cggcatggat
      481 accgggcatc ttgatgttcc cgacggtctt ttttgacctg taagttgtta cgaaagagca
      541 agacagatgg ccaccaccat caactttcag tgccatatga gtgtatcccc tcagaccacc
      601 atctgctgga tacatcatct ctgtattagg ctcccaaccc ttggtcttct tctgcatcac
      661 gggaccattg gagggaaagt ttacccctct gacttggacg tggtaaacga gacagccatc
      721 ctcaaggctg gtgtcctgca tgacggtgac gactccacca tcttcgtatc tcgtaactct
      781 ttcccaagta aaaccctcag gaaaggactg tttaaagaaa tcaggaatgc ctttcgggta
      841 cttgataaaa gtacggctgc catacatgaa cgacgtggca agaatgtcaa aggcaaatgg
      901 caggggtcct ccctcgatga ctttgatcct catggtttga gttcccatgt acggattgcc
      961 ttctccttca cctgtgcatt tgaattggtg gccgttgacc gaaccttcca tgaccacctt
     1021 catacgcata ttttccttga tcagctcttc gcc
//
