LOCUS       sequence-140280-       15926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Wang SC, Shao R, Pao AY, Zhang S, Hung MC, Su LK
  TITLE     Inhibition of cancer cell growth by BRCA2.
  JOURNAL   Cancer Res. 2002 Mar 1. 62(5):1311-4.
  PUBMED    11888897
REFERENCE   2  (bases 1 to 15926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2002 Mar 1. 62(5):1311-4."
COMMENT     Sequence Label: pcDNA3 236HSC WT (BRCA2)
FEATURES             Location/Qualifiers
     source          1..15926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(993..1784,1785..1853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1616..1635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1854..1958)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2033..2052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2224..2603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2604..2807
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2758..2778
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2852..2871
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2852..2870
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(13468..13486)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(13469..13486)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(13506..13523)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13512..13736
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13782..14210
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13869..13888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14079..14100
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(14219..14239)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14224..14553
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14404..14539
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14466..14485
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             14620..15414
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14674..14693)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     15284..15303
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    15588..15709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15625..15644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15679..15698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15758..15774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15758..15774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15771..15793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15782..15798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15806..15812,15813..15830,15831..15836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15851..15872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     2041 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     2101 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     2161 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     2221 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2281 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2341 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2401 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2461 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2521 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2581 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2641 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2701 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2761 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
     2821 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
     2881 cttggtaccg gtggcgcgag cttctgaaac taggcggcag aggcggagcc gctgtggcac
     2941 tgctgcgcct ctgctgcgcc tcgggtgtct tttgcggcgg tgggtcgccg ccgggagaag
     3001 cgtgagggga cagatttgtg accggcgcgg tttttgtcag cttactccgg ccaaaaaaga
     3061 actgcgcctc tggagcggac ttatttacca agcattggag gaatatcgta gttgcggccg
     3121 ctcgaggtaa aaatgcctat tggatccaaa gagaggccaa cattttttga aatttttaag
     3181 acacgctgca acaaagcaga tttaggacca ataagtctta attggtttga agaactttct
     3241 tcagaagctc caccctataa ttctgaacct gcagaagaat ctgaacataa aaacaacaat
     3301 tacgaaccaa acctatttaa aactccacaa aggaaaccat cttataatca gctggcttca
     3361 actccaataa tattcaaaga gcaagggctg actctgccgc tgtaccaatc tcctgtaaaa
     3421 gaattagata aattcaaatt agacttagga aggaatgttc ccaatagtag acataaaagt
     3481 cttcgcacag tgaaaactaa aatggatcaa gcagatgatg tttcctgtcc acttctaaat
     3541 tcttgtctta gtgaaagtcc tgttgttcta caatgtacac atgtaacacc acaaagagat
     3601 aagtcagtgg tatgtgggag tttgtttcat acaccaaagt ttgtgaaggg tcgtcagaca
     3661 ccaaaacata tttctgaaag tctaggagct gaggtggatc ctgatatgtc ttggtcaagt
     3721 tctttagcta caccacccac ccttagttct actgtgctca tagtcagaaa tgaagaagca
     3781 tctgaaactg tatttcctca tgatactact gctaatgtga aaagctattt ttccaatcat
     3841 gatgaaagtc tgaagaaaaa tgatagattt atcgcttctg tgacagacag tgaaaacaca
     3901 aatcaaagag aagctgcaag tcatggattt ggaaaaacat cagggaattc atttaaagta
     3961 aatagctgca aagaccacat tggaaagtca atgccaaatg tcctagaaga tgaagtatat
     4021 gaaacagttg tagatacctc tgaagaagat agtttttcat tatgtttttc taaatgtaga
     4081 acaaaaaatc tacaaaaagt aagaactagc aagactagga aaaaaatttt ccatgaagca
     4141 aacgctgatg aatgtgaaaa atctaaaaac caagtgaaag aaaaatactc atttgtatct
     4201 gaagtggaac caaatgatac tgatccatta gattcaaatg tagcaaatca gaagcccttt
     4261 gagagtggaa gtgacaaaat ctccaaggaa gttgtaccgt ctttggcctg tgaatggtct
     4321 caactaaccc tttcaggtct aaatggagcc cagatggaga aaatacccct attgcatatt
     4381 tcttcatgtg accaaaatat ttcagaaaaa gacctattag acacagagaa caaaagaaag
     4441 aaagattttc ttacttcaga gaattctttg ccacgtattt ctagcctacc aaaatcagag
     4501 aagccattaa atgaggaaac agtggtaaat aagagagatg aagagcagca tcttgaatct
     4561 catacagact gcattcttgc agtaaagcag gcaatatctg gaacttctcc agtggcttct
     4621 tcatttcagg gtatcaaaaa gtctatattc agaataagag aatcacctaa agagactttc
     4681 aatgcaagtt tttcaggtca tatgactgat ccaaacttta aaaaagaaac tgaagcctct
     4741 gaaagtggac tggaaataca tactgtttgc tcacagaagg aggactcctt atgtccaaat
     4801 ttaattgata atggaagctg gccagccacc accacacaga attctgtagc tttgaagaat
     4861 gcaggtttaa tatccacttt gaaaaagaaa acaaataagt ttatttatgc tatacatgat
     4921 gaaacatctt ataaaggaaa aaaaataccg aaagaccaaa aatcagaact aattaactgt
     4981 tcagcccagt ttgaagcaaa tgcttttgaa gcaccactta catttgcaaa tgctgattca
     5041 ggtttattgc attcttctgt gaaaagaagc tgttcacaga atgattctga agaaccaact
     5101 ttgtccttaa ctagctcttt tgggacaatt ctgaggaaat gttctagaaa tgaaacatgt
     5161 tctaataata cagtaatctc tcaggatctt gattataaag aagcaaaatg taataaggaa
     5221 aaactacagt tatttattac cccagaagct gattctctgt catgcctgca ggaaggacag
     5281 tgtgaaaatg atccaaaaag caaaaaagtt tcagatataa aagaagaggt cttggctgca
     5341 gcatgtcacc cagtacaaca ttcaaaagtg gaatacagtg atactgactt tcaatcccag
     5401 aaaagtcttt tatatgatca tgaaaatgcc agcactctta ttttaactcc tacttccaag
     5461 gatgttctgt caaacctagt catgatttct agaggcaaag aatcatacaa aatgtcagac
     5521 aagcttaaag gtaacaatta tgaatctgat gttgaattaa ccaaaaatat tcccatggaa
     5581 aagaatcaag atgtatgtgc tttaaatgaa aattataaaa acgttgagct gttgccacct
     5641 gaaaaataca tgagagtagc atcaccttca agaaaggtac aattcaacca aaacacaaat
     5701 ctaagagtaa tccaaaaaaa tcaagaagaa actacttcaa tttcaaaaat aactgtcaat
     5761 ccagactctg aagaactttt ctcagacaat gagaataatt ttgtcttcca agtagctaat
     5821 gaaaggaata atcttgcttt aggaaatact aaggaacttc atgaaacaga cttgacttgt
     5881 gtaaacgaac ccattttcaa gaactctacc atggttttat atggagacac aggtgataaa
     5941 caagcaaccc aagtgtcaat taaaaaagat ttggtttatg ttcttgcaga ggagaacaaa
     6001 aatagtgtaa agcagcatat aaaaatgact ctaggtcaag atttaaaatc ggacatctcc
     6061 ttgaatatag ataaaatacc agaaaaaaat aatgattaca tgaacaaatg ggcaggactc
     6121 ttaggtccaa tttcaaatca cagttttgga ggtagcttca gaacagcttc aaataaggaa
     6181 atcaagctct ctgaacataa cattaagaag agcaaaatgt tcttcaaaga tattgaagaa
     6241 caatatccta ctagtttagc ttgtgttgaa attgtaaata ccttggcatt agataatcaa
     6301 aagaaactga gcaagcctca gtcaattaat actgtatctg cacatttaca gagtagtgta
     6361 gttgtttctg attgtaaaaa tagtcatata acccctcaga tgttattttc caagcaggat
     6421 tttaattcaa accataattt aacacctagc caaaaggcag aaattacaga actttctact
     6481 atattagaag aatcaggaag tcagtttgaa tttactcagt ttagaaagcc aagctacata
     6541 ttgcagaaga gtacatttga agtgcctgaa aaccagatga ctatcttaaa gaccacttct
     6601 gaggaatgca gagatgctga tcttcatgtc ataatgaatg ccccatcgat tggtcaggta
     6661 gacagcagca agcaatttga aggtacagtt gaaattaaac ggaagtttgc tggcctgttg
     6721 aaaaatgact gtaacaaaag tgcttctggt tatttaacag atgaaaatga agtggggttt
     6781 aggggctttt attctgctca tggcacaaaa ctgaatgttt ctactgaagc tctgcaaaaa
     6841 gctgtgaaac tgtttagtga tattgagaat attagtgagg aaacttctgc agaggtacat
     6901 ccaataagtt tatcttcaag taaatgtcat gattctgttg tttcaatgtt taagatagaa
     6961 aatcataatg ataaaactgt aagtgaaaaa aataataaat gccaactgat attacaaaat
     7021 aatattgaaa tgactactgg cacttttgtt gaagaaatta ctgaaaatta caagagaaat
     7081 actgaaaatg aagataacaa atatactgct gccagtagaa attctcataa cttagaattt
     7141 gatggcagtg attcaagtaa aaatgatact gtttgtattc ataaagatga aacggacttg
     7201 ctatttactg atcagcacaa catatgtctt aaattatctg gccagtttat gaaggaggga
     7261 aacactcaga ttaaagaaga tttgtcagat ttaacttttt tggaagttgc gaaagctcaa
     7321 gaagcatgtc atggtaatac ttcaaataaa gaacagttaa ctgctactaa aacggagcaa
     7381 aatataaaag attttgagac ttctgataca ttttttcaga ctgcaagtgg gaaaaatatt
     7441 agtgtcgcca aagagtcatt taataaaatt gtaaatttct ttgatcagaa accagaagaa
     7501 ttgcataact tttccttaaa ttctgaatta cattctgaca taagaaagaa caaaatggac
     7561 attctaagtt atgaggaaac agacatagtt aaacacaaaa tactgaaaga aagtgtccca
     7621 gttggtactg gaaatcaact agtgaccttc cagggacaac ccgaacgtga tgaaaagatc
     7681 aaagaaccta ctctgttggg ttttcataca gctagcggga aaaaagttaa aattgcaaag
     7741 gaatctttgg acaaagtgaa aaaccttttt gatgaaaaag agcaaggtac tagtgaaatc
     7801 accagtttta gccatcaatg ggcaaagacc ctaaagtaca gagaggcctg taaagacctt
     7861 gaattagcat gtgagaccat tgagatcaca gctgccccaa agtgtaaaga aatgcagaat
     7921 tctctcaata atgataaaaa ccttgtttct attgagactg tggtgccacc taagctctta
     7981 agtgataatt tatgtagaca aactgaaaat ctcaaaacat caaaaagtat ctttttgaaa
     8041 gttaaagtac atgaaaatgt agaaaaagaa acagcaaaaa gtcctgcaac ttgttacaca
     8101 aatcagtccc cttattcagt cattgaaaat tcagccttag ctttttacac aagttgtagt
     8161 agaaaaactt ctgtgagtca gacttcatta cttgaagcaa aaaaatggct tagagaagga
     8221 atatttgatg gtcaaccaga aagaataaat actgcagatt atgtaggaaa ttatttgtat
     8281 gaaaataatt caaacagtac tatagctgaa aatgacaaaa atcatctctc cgaaaaacaa
     8341 gatacttatt taagtaacag tagcatgtct aacagctatt cctaccattc tgatgaggta
     8401 tataatgatt caggatatct ctcaaaaaat aaacttgatt ctggtattga gccagtattg
     8461 aagaatgttg aagatcaaaa aaacactagt ttttccaaag taatatccaa tgtaaaagat
     8521 gcaaatgcat acccacaaac tgtaaatgaa gatatttgcg ttgaggaact tgtgactagc
     8581 tcttcaccct gcaaaaataa aaatgcagcc attaaattgt ccatatctaa tagtaataat
     8641 tttgaggtag ggccacctgc atttaggata gccagtggta aaatcgtttg tgtttcacat
     8701 gaaacaatta aaaaagtgaa agacatattt acagacagtt tcagtaaagt aattaaggaa
     8761 aacaacgaga ataaatcaaa aatttgccaa acgaaaatta tggcaggttg ttacgaggca
     8821 ttggatgatt cagaggatat tcttcataac tctctagata atgatgaatg tagcacgcat
     8881 tcacataagg tttttgctga cattcagagt gaagaaattt tacaacataa ccaaaatatg
     8941 tctggattgg agaaagtttc taaaatatca ccttgtgatg ttagtttgga aacttcagat
     9001 atatgtaaat gtagtatagg gaagcttcat aagtcagtct catctgcaaa tacttgtggg
     9061 atttttagca cagcaagtgg aaaatctgtc caggtatcag atgcttcatt acaaaacgca
     9121 agacaagtgt tttctgaaat agaagatagt accaagcaag tcttttccaa agtattgttt
     9181 aaaagtaacg aacattcaga ccagctcaca agagaagaaa atactgctat acgtactcca
     9241 gaacatttaa tatcccaaaa aggcttttca tataatgtgg taaattcatc tgctttctct
     9301 ggatttagta cagcaagtgg aaagcaagtt tccattttag aaagttcctt acacaaagtt
     9361 aagggagtgt tagaggaatt tgatttaatc agaactgagc atagtcttca ctattcacct
     9421 acgtctagac aaaatgtatc aaaaatactt cctcgtgttg ataagagaaa cccagagcac
     9481 tgtgtaaact cagaaatgga aaaaacctgc agtaaagaat ttaaattatc aaataactta
     9541 aatgttgaag gtggttcttc agaaaataat cactctatta aagtttctcc atatctctct
     9601 caatttcaac aagacaaaca acagttggta ttaggaacca aagtctcact tgttgagaac
     9661 attcatgttt tgggaaaaga acaggcttca cctaaaaacg taaaaatgga aattggtaaa
     9721 actgaaactt tttctgatgt tcctgtgaaa acaaatatag aagtttgttc tacttactcc
     9781 aaagattcag aaaactactt tgaaacagaa gcagtagaaa ttgctaaagc ttttatggaa
     9841 gatgatgaac tgacagattc taaactgcca agtcatgcca cacattctct ttttacatgt
     9901 cccgaaaatg aggaaatggt tttgtcaaat tcaagaattg gaaaaagaag aggagagccc
     9961 cttatcttag tgggagaacc ctcaatcaaa agaaacttat taaatgaatt tgacaggata
    10021 atagaaaatc aagaaaaatc cttaaaggct tcaaaaagca ctccagatgg cacaataaaa
    10081 gatcgaagat tgtttatgca tcatgtttct ttagagccga ttacctgtgt accctttcgc
    10141 acaactaagg aacgtcaaga gatacagaat ccaaatttta ccgcacctgg tcaagaattt
    10201 ctgtctaaat ctcatttgta tgaacatctg actttggaaa aatcttcaag caatttagca
    10261 gtttcaggac atccatttta tcaagtttct gctacaagaa atgaaaaaat gagacacttg
    10321 attactacag gcagaccaac caaagtcttt gttccacctt ttaaaactaa atcacatttt
    10381 cacagagttg aacagtgtgt taggaatatt aacttggagg aaaacagaca aaagcaaaac
    10441 attgatggac atggctctga tgatagtaaa aataagatta atgacaatga gattcatcag
    10501 tttaacaaaa acaactccaa tcaagcagca gctgtaactt tcacaaagtg tgaagaagaa
    10561 cctttagatt taattacaag tcttcagaat gccagagata tacaggatat gcgaattaag
    10621 aagaaacaaa ggcaacgcgt ctttccacag ccaggcagtc tgtatcttgc aaaaacatcc
    10681 actctgcctc gaatctctct gaaagcagca gtaggaggcc aagttccctc tgcgtgttct
    10741 cataaacagc tgtatacgta tggcgtttct aaacattgca taaaaattaa cagcaaaaat
    10801 gcagagtctt ttcagtttca cactgaagat tattttggta aggaaagttt atggactgga
    10861 aaaggaatac agttggctga tggcggatgg ctcataccct ccaatgatgg aaaggctgga
    10921 aaagaagaat tttatagggc tctgtgtgac actccaggtg tggatccaaa gcttatttct
    10981 agaatttggg tttataatca ctatagatgg atcatatgga aactggcagc tatggaatgt
    11041 gcctttccta aggaatttgc taatagatgc ctaagcccag aaagggtgct tcttcaacta
    11101 aaatacagat atgatacgga aattgataga agcagaagat cggctataaa aaagataatg
    11161 gaaagggatg acacagctgc aaaaacactt gttctctgtg tttctgacat aatttcattg
    11221 agcgcaaata tatctgaaac ttctagcaat aaaactagta gtgcagatac ccaaaaagtg
    11281 gccattattg aacttacaga tgggtggtat gctgttaagg cccagttaga tcctcccctc
    11341 ttagctgtct taaagaatgg cagactgaca gttggtcaga agattattct tcatggagca
    11401 gaactggtgg gctctcctga tgcctgtaca cctcttgaag ccccagaatc tcttatgtta
    11461 aagatttctg ctaacagtac tcggcctgct cgctggtata ccaaacttgg gttctttcct
    11521 gaccctagac cttttcctct gcccttatca tcgcttttca gtgatggagg aaatgttggt
    11581 tgtgttgatg taattattca aagagcatac cctatacagt ggatggagaa gacatcatct
    11641 ggattataca tatttcgcaa tgaaagagag gaagaaaagg aagcagcaaa atatgtggag
    11701 gcccaacaaa agagactaga agccttattc actaaaattc aggaggaatt tgaagaacat
    11761 gaagaaaaca caacaaaacc atatttacca tcacgtgcac taacaagaca gcaagttcgt
    11821 gctttgcaag atggtgcaga gctttatgaa gcagtgaaga atgcagcaga cccagcttac
    11881 cttgagggtt atttcagtga agagcagtta agagccttga ataatcacag gcaaatgttg
    11941 aatgataaga aacaagctca gatccagttg gaaattagga aggccatgga atctgctgaa
    12001 caaaaggaac aaggtttatc aagggatgtc acaaccgtgt ggaagttgcg tattgtaagc
    12061 tattcaaaaa aagaaaaaga ttcagttata ctgagtattt ggcgtccatc atcagattta
    12121 tattctctgt taacagaagg aaagagatac agaatttatc atcttgcaac ttcaaaatct
    12181 aaaagtaaat ctgaaagagc taacatacag ttagcagcga caaaaaaaac tcagtatcaa
    12241 caactaccgg tttcagatga aattttattt cagatttacc agccacggga gccccttcac
    12301 ttcagcaaat ttttagatcc agactttcag ccatcttgtt ctgaggtgga cctaatagga
    12361 tttgtcgttt ctgttgtgaa aaaaacagga cttgcccctt tcgtctattt gtcagacgaa
    12421 tgttacaatt tactggcaat aaagttttgg atagacctta atgaggacat tattaagcct
    12481 catatgttaa ttgctgcaag caacctccag tggcgaccag aatccaaatc aggccttctt
    12541 actttatttg ctggagattt ttctgtgttt tctgctagtc caaaagaggg ccactttcaa
    12601 gagacattca acaaaatgaa aaatactgtt gagaatattg acatactttg caatgaagca
    12661 gaaaacaagc ttatgcatat actgcatgca aatgatccca agtggtccac cccaactaaa
    12721 gactgtactt cagggccgta cactgctcaa atcattcctg gtacaggaaa caagcttctg
    12781 atgtcttctc ctaattgtga gatatattat caaagtcctt tatcactttg tatggccaaa
    12841 aggaagtctg tttccacacc tgtctcagcc cagatgactt caaagtcttg taaaggggag
    12901 aaagagattg atgaccaaaa gaactgcaaa aagagaagag ccttggattt cttgagtaga
    12961 ctgcctttac ctccacctgt tagtcccatt tgtacatttg tttctccggc tgcacagaag
    13021 gcatttcagc caccaaggag ttgtggcacc aaatacgaaa cacccataaa gaaaaaagaa
    13081 ctgaattctc ctcagatgac tccatttaaa aaattcaatg aaatttctct tttggaaagt
    13141 aattcaatag ctgacgaaga acttgcattg ataaataccc aagctctttt gtctggttca
    13201 acaggagaaa aacaatttat atctgtcagt gaatccacta ggactgctcc caccagttca
    13261 gaagattatc tcagactgaa acgacgttgt actacatctc tgatcaaaga acaggagagt
    13321 tcccaggcca gtacggaaga atgtgagaaa aataagcagg acacaattac aactaaaaaa
    13381 tatatctaag catttgcaaa ggcgacaata aattattgac gcttaacctt tccagtttat
    13441 ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
    13501 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
    13561 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
    13621 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
    13681 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
    13741 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
    13801 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    13861 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    13921 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    13981 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    14041 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    14101 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
    14161 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
    14221 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    14281 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
    14341 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
    14401 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
    14461 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
    14521 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
    14581 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
    14641 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
    14701 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
    14761 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
    14821 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
    14881 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
    14941 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
    15001 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
    15061 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
    15121 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
    15181 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
    15241 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
    15301 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
    15361 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
    15421 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
    15481 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    15541 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
    15601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    15661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
    15721 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    15781 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    15841 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    15901 agtcgggaaa cctgtcgtgc cagctg
//
