LOCUS       sequence-140296-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nabi2.216 is a FRET (Forster Resonance Energy Transfer)-based 
            protein voltage sensor. This probe contains Clover and mRuby2 
            inserted at different locations in the Ciona voltage sensitive 
            domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Sung U, Sepehri-Rad M, Piao HH, Jin L, Hughes T, Cohen LB, Baker BJ
  TITLE     Developing Fast Fluorescent Protein Voltage Sensors by Optimizing 
            FRET Interactions.
  JOURNAL   PLoS One. 2015 Nov 20;10(11):e0141585. doi: 
            10.1371/journal.pone.0141585. eCollection 2015.
  PUBMED    26587834
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-11-20"; volume: "10(11):e0141585. doi"; pages: " 
            10.1371/journal.pone.0141585. eCollection 2015"
COMMENT     Sequence Label: Nabi2.216
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(400..421)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1008..1029
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttatggaggg attcgacggt
      121 tcagatttta gtcctccagc tgatttagtt ggcgttgacg gtgcagtcat gcggaacgtc
      181 gttgacgtca cgataaatgg tgacgtcact gctccgccga aagctgcgcc aagaaaaagt
      241 gaatcggtaa agaaagttca ttggaatgat gtagaccaag gaccgagtga aaaaccagag
      301 acaagacagg aggaacgaat agatataccc gagatttcag gtctatggtg gggcatggtg
      361 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      421 gtaaacggcc acaagttcag cgtccgcggc gagggcgagg gcgatgccac caacggcaag
      481 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      541 accaccttcg gctacggcgt ggcctgcttc agccgctacc ccgaccacat gaagcagcac
      601 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat ctctttcaag
      661 gacgacggta cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      721 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      781 gagtacaact tcaacagcca caacgtctat atcacggccg acaagcagaa gaacggcatc
      841 aaggctaact tcaagatccg ccacaacgtt gaggacggca gcgtgcagct cgccgaccac
      901 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      961 agccatcagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1021 gagttcgtga ccgccgccga gaa
//
