LOCUS       sequence-140300-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nabi2.216 is a FRET (Forster Resonance Energy Transfer)-based 
            protein voltage sensor. This probe contains Clover and mRuby2 
            inserted at different locations in the Ciona voltage sensitive 
            domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Sung U, Sepehri-Rad M, Piao HH, Jin L, Hughes T, Cohen LB, Baker BJ
  TITLE     Developing Fast Fluorescent Protein Voltage Sensors by Optimizing 
            FRET Interactions.
  JOURNAL   PLoS One. 2015 Nov 20;10(11):e0141585. doi: 
            10.1371/journal.pone.0141585. eCollection 2015.
  PUBMED    26587834
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-11-20"; volume: "10(11):e0141585. doi"; pages: " 
            10.1371/journal.pone.0141585. eCollection 2015"
COMMENT     Sequence Label: Nabi2.216
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(613..634)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtacggattg ccttctcctt cacctgtgca tttgaattgg tggccgttga ccgaaccttc
       61 catgaccacc ttcatacgca tattttcctt gatcagctct tcgcccttag acaccattct
      121 tctgcttgaa gccttcattt gttggtggga ataaaatatt cttgctaatc gaaccacacg
      181 cagcaaacgg gccaacacaa ccaattgccc caaaccatcg gctcctgttt cttgcacgta
      241 ttcatctaac acagtgtaaa atatcgtgac gacgaatgtg acaacgataa tcaaaccatc
      301 agcaacctcc caggggttgg tgaagaaatt cttgggcccg taggcaaata tccttaatcc
      361 taaatccagc atgaaataac aagaaagagc caaagccatc ccgtcataaa aggattgtga
      421 agattcactt tttcctggaa gactgagatc aatgatcatg aggatgatgt ccaagaaaat
      481 taggaagact ccaaagactc gcatccctag atgatcaatc actgctcgga cacgaaactg
      541 gacgcgacct acaccagtag taggtatctc cattctccca tcgtccactc catgttcatt
      601 ctcggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      661 gctcagggcg gactgatggc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      721 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct caacgttgtg
      781 gcggatcttg aagttagcct tgatgccgtt cttctgcttg tcggccgtga tatagacgtt
      841 gtggctgttg aagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      901 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      961 ggtcttgtag gtaccgtcgt ccttgaaaga
//
