LOCUS       sequence-140307-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nabi2.242 is a FRET (Forster Resonance Energy Transfer)-based 
            protein voltage sensor. This probe contains Clover and mRuby2 
            inserted at different locations in the Ciona voltage sensitive 
            domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Sung U, Sepehri-Rad M, Piao HH, Jin L, Hughes T, Cohen LB, Baker BJ
  TITLE     Developing Fast Fluorescent Protein Voltage Sensors by Optimizing 
            FRET Interactions.
  JOURNAL   PLoS One. 2015 Nov 20;10(11):e0141585. doi: 
            10.1371/journal.pone.0141585. eCollection 2015.
  PUBMED    26587834
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-11-20"; volume: "10(11):e0141585. doi"; pages: " 
            10.1371/journal.pone.0141585. eCollection 2015"
COMMENT     Sequence Label: Nabi2.242
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    113..337
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     326..343
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(356..1066)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
       61 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag
      121 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
      181 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
      241 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
      301 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg agggcgcgcc tattacttgt
      361 acagctcgtc catcccacca ccaagcccgg cgaacttggc aactgcgtgt tcgcgttgta
      421 ctacgaacat ttcattgtca ctttcctcta acctttccag gcggtgatca acggcatgga
      481 taccgggcat cttgatgttc ccgacggtct tttttgacct gtaagttgtt acgaaagagc
      541 aagacagatg gccaccacca tcaactttca gtgccatatg agtgtatccc ctcagaccac
      601 catctgctgg atacatcatc tctgtattag gctcccaacc cttggtcttc ttctgcatca
      661 cgggaccatt ggagggaaag tttacccctc tgacttggac gtggtaaacg agacagccat
      721 cctcaaggct ggtgtcctgc atgacggtga cgactccacc atcttcgtat ctcgtaactc
      781 tttcccaagt aaaaccctca ggaaaggact gtttaaagaa atcaggaatg cctttcgggt
      841 acttgataaa agtacggctg ccatacatga acgacgtggc aagaatgtca aaggcaaatg
      901 gcaggggtcc tccctcgatg actttgatcc tcatggtttg agttcccatg tacggattgc
      961 cttctccttc acctgtgcat ttgaattggt ggccgttgac cgaaccttcc atgaccacct
     1021 tcatacgcat attttccttg atcagctctt cgcccttaga caccattctt ctgcttgaag
     1081 ccttcatt
//
