LOCUS       sequence-140310-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nabi2.244 is a FRET (Forster Resonance Energy Transfer)-based 
            protein voltage sensor. This probe contains Clover and mRuby2 
            inserted at different locations in the Ciona voltage sensitive 
            domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Sung U, Sepehri-Rad M, Piao HH, Jin L, Hughes T, Cohen LB, Baker BJ
  TITLE     Developing Fast Fluorescent Protein Voltage Sensors by Optimizing 
            FRET Interactions.
  JOURNAL   PLoS One. 2015 Nov 20;10(11):e0141585. doi: 
            10.1371/journal.pone.0141585. eCollection 2015.
  PUBMED    26587834
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-11-20"; volume: "10(11):e0141585. doi"; pages: " 
            10.1371/journal.pone.0141585. eCollection 2015"
COMMENT     Sequence Label: Nabi2.244
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(433..454)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1041..1062
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttatggaggg attcgacggt
      121 tcagatttta gtcctccagc tgatttagtt ggcgttgacg gtgcagtcat gcggaacgtc
      181 gttgacgtca cgataaatgg tgacgtcact gctccgccga aagctgcgcc aagaaaaagt
      241 gaatcggtaa agaaagttca ttggaatgat gtagaccaag gaccgagtga aaaaccagag
      301 acaagacagg aggaacgaat agatataccc gagatttcag gtctatggtg gggcgagaat
      361 gaacatggag tggacgatgg gagaatgatg gtgagcaagg gcgaggagct gttcaccggg
      421 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc
      481 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc
      541 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cgtggcctgc
      601 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      661 ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc
      721 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      781 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaacgtc
      841 tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagat ccgccacaac
      901 gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      961 ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccgccct gagcaaagac
     1021 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgacc
//
