LOCUS       sequence-140324-        3879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of MDR3 in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3879)
  TITLE     Nakamoto lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFastBacI/MDR3cysless
FEATURES             Location/Qualifiers
     source          1..3879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3841..3870
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ctgcagatgg aacttgaaga ggaccttaag ggaagagcag acaagaactt ctcaaagatg
       61 ggcaaaaaga gtaaaaagga gaagaaagaa aagaaaccag cagtcagtgt gcttacaatg
      121 tttcgttatg caggttggct ggacaggttg tacatgctgg tgggaactct ggctgctatt
      181 atccatggag tggcgctccc acttatgatg ctgatctttg gtgacatgac agatagcttt
      241 gcaagtgtag gaaacgtctc taaaaacagt actaatatga gtgaggccga taaaagagcc
      301 atgtttgcca aactggagga agaaatgacc acgtacgcct actattacac cgggattggt
      361 gctggtgtgc tcatagttgc ctacatccag gtttcatttt gggctttggc agctggaaga
      421 cagatacaca agatcaggca gaagtttttt catgctataa tgaatcagga gataggctgg
      481 tttgatgtgc atgacgttgg ggagctcaac acccggctca cagatgatgt ttccaaaatt
      541 aatgaaggaa ttggtgacaa aatcggaatg ttcttccagg caatggcaac attttttggt
      601 ggttttataa taggatttac ccgtggctgg aagctaaccc ttgtgatttt ggccatcagc
      661 cctgttcttg gactgtcagc tggtatttgg gcaaagatat tgtcttcatt tactgataag
      721 gaactccatg cttatgcaaa agctggagca gttgctgaag aagtcttagc agccatcaga
      781 actgtgattg cgtttggagg acaaaagaag gaacttgaaa ggtacaataa caacttggaa
      841 gaagctaaaa ggctggggat aaagaaagct atcacggcca acatctccat gggtgcagct
      901 tttctcctta tctatgcatc atatgctctg gcattctggt atgggacttc cttggtcatc
      961 tccaaagaat actctattgg acaagtgctc actgtcttct tttccgtgtt aattggagca
     1021 ttcagtgttg gacaggcatc tccaaatatt gaagccttcg ccaatgcacg aggagcagct
     1081 tatgaagtct tcaaaataat tgataataag cccagtatag acagcttctc aaagagtggg
     1141 cacaaaccag acaacataca aggaaatctg gaatttaaga atattcactt cagttaccca
     1201 tcgcgaaaag aagttcagat cttgaagggc ctcaatctga aggtgaagag cggacagacg
     1261 gtggccctgg ttggcaacag tggcgccgga aaaagcacaa ctgtccagct gatgcaaagg
     1321 ctctacgacc ccctagatgg catggtcagt atcgacggac aggacatcag aaccatcaat
     1381 gtgaggtatc tgagggagat cattggtgtg gtgagtcagg aacctgtgct gtttgccacc
     1441 acgatcgccg agaacattcg ctatggccga gaagatgtca ccatggatga gattgagaaa
     1501 gctgtcaagg aagccaatgc ctatgacttc atcatgaaac tgccccacca atttgacacc
     1561 ctggttggtg agagaggggc gcagctgagt gggggacaga aacagagaat cgccattgcc
     1621 cgggccctgg tccgcaatcc caagatcctt ttgttggacg aggccacctc agccctggat
     1681 acagaaagtg aagctgtggt tcaggccgca ctggataagg ctagagaagg ccggaccacc
     1741 attgtgatag ctcatcgctt gtcgaccgtt cgtaatgctg acgtcattgc tggttttgat
     1801 ggtggtgtca ttgtggagca aggaaatcat gatgagctca tgagagaaaa gggcatttac
     1861 ttcaaacttg tcatgacaca gacagcagga aatgaaattg aattaggaaa tgaagctgct
     1921 aaatctaagg atgaaattga taatttagac atgtcttcaa aagattcagg atccagtcta
     1981 ataagaagaa gatcaactcg caaaagcatc gccggcccac atgaccaaga caggaagctt
     2041 agtaccaaag aggccctgga tgaagatgta cctccagctt ccttttggcg gatcctgaag
     2101 ttgaattcaa ctgaatggcc ttattttgtg gttggtatat tcgcagccat aataaatgga
     2161 ggcttacagc cagcattctc cgtaatattt tcaaaagttg taggggtttt tacaaatggt
     2221 ggcccccctg aaacccagcg gcagaacagc aacttgtttt ccttgttgtt tctgatcctt
     2281 gggatcattt ctttcattac attttttctt cagggcttca catttggcaa agctggagag
     2341 attctcacca agcgactccg atacatggtt ttcaaatcca tgctgagaca ggatgtgagc
     2401 tggtttgatg accctaaaaa caccaccgga gcactgacca ccaggctcgc caacgatgct
     2461 gctcaagtga aaggggctac agggagtcgg cttgctgtga ttttccagaa catagcaaat
     2521 cttgggacag gaatcatcat atccctaatc tatggctggc aactaacact tttactctta
     2581 gcaattgtac ccatcattgc gatagcagga gtggttgaaa tgaaaatgtt gtctggacaa
     2641 gcactgaaag ataagaagga actagaaggt tctggaaaga ttgctacgga agcaattgaa
     2701 aacttccgca ctgttgtctc tttgactcgc gagcagaagt ttgaaaccat gtatgcccag
     2761 agcttgcaga taccatacag aaatgcgatg aagaaagcac acgtgtttgg gatcacgttc
     2821 tccttcaccc aggccatgat gtatttttct tatgctgctg ctttccggtt cggtgcctac
     2881 ttggtgacac aacaactcat gacttttgaa aatgttctac tagtattctc agctattgtc
     2941 tttggtgcca tggcagtggg gcaggtcagt tcattcgctc ctgactatgc gaaagccaca
     3001 gtgtcagcat cccacatcat caggatcatt gagaaaaccc ccgagattga cagctacagc
     3061 acgcaaggcc taaagccgaa tatgttggaa ggaaatgtgc aatttagtgg agtcgtgttc
     3121 aactatccca cccgacccag catcccagtg cttcaggggc tgagccttga ggtgaagaag
     3181 ggccagacgc tggccctggt gggcagcagt ggcgccggga agagcacagt ggtccagctg
     3241 ctcgagcgct tctacgaccc catggctgga tcagtgtttc tagatggcaa agaaataaag
     3301 caactgaatg tccagtggct ccgagcacag ctgggcattg tgtcccaaga gcccattctc
     3361 tttgacgcta gcatcgcaga gaacattgcc tacggagaca acagtcgggt cgtgtcttat
     3421 gaggagattg tgagggcagc caaggaggcc aacatccacc agttcatcga ctcgctacct
     3481 gataaataca acaccagagt aggagacaaa ggcactcagc tgtcgggtgg gcagaagcag
     3541 cgcatcgcca tcgcacgcgc cctcgtcaga cagcctcaca ttttacttct ggacgaagca
     3601 acatcagctc tggatacaga aagtgaaaag gttgtccagg aagcgctgga caaagccagg
     3661 gaaggccgca ccgcaattgt gatcgctcac cgcctgtcca ccatccagaa cgcggacttg
     3721 atcgtggtga ttcagaacgg caaggtcaag gagcacggca cccaccagca gctgctggcg
     3781 cagaagggca tctacttctc aatggtcagt gtgcaggctg gagcaaagcg ctcatacgta
     3841 catcaccatc accatcacca ccaccatcat tgaggtacc
//
