LOCUS       sequence-140327-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of SERCA in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  TITLE     Nakamoto lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAGHis10pFastBacHTA/Serca1
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(115..120,121..137,138..143))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        495..586
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     511..528
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             653..676
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             677..706
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             728..748
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
       61 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
      121 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
      181 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag
      241 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc
      301 aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg
      361 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc
      421 gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct acgtatactc
      481 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
      541 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
      601 ataccgtccc accatcgggc gcggatctcg gtccgaaacc atgtcgtact acgattacaa
      661 ggatgacgac gataagcatc accatcacca tcaccatcac catcacgatt acgatatccc
      721 aacgaccgaa aacctgtatt ttcagggcgc catggat
//
