LOCUS       sequence-140339-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 
            of Arrestin3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash2
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    553..674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(590..609)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     644..663
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(818..839)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccataacccc gctgctcagt gacaaccgag agaagcgtgg ccttgccctt gatgggcaac
       61 tcaagcacga agacaccaac ctggcttcca gcaccattgt gaaggaggga gccaacaagg
      121 aggtgctggg aatcctagta tcctacaggg tcaaggtgaa gctggtggtg tctcgaggcg
      181 gggatgtctc cgtggagcta cctttcgtcc taatgcaccc caagccccac gaccacatca
      241 cccttccccg accccagtca gccccccggg aaatagacat ccctgtggat accaacctca
      301 ttgaattcga taccaactat gccacagacg acgacatcgt gtttgaggac tttgcgaggc
      361 ttcggctgaa ggggatgaag gatgacgact gtgatgacca gttctgctag tctagagggc
      421 ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
      481 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      541 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      601 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
      661 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
      721 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
      781 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
      841 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
      901 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
      961 tcggaaccct aaagggagcc cccgattta
//
