LOCUS       sequence-140343-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSIREN-RetroQ vector containing control sequence N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, 
            Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic
            B, Dumur CI, Landry JW
  TITLE     BPTF inhibits NK cell activity and the abundance of natural 
            cytotoxicity receptor co-ligands.
  JOURNAL   Oncotarget. 2017 May 12;8(38):64344-64357. doi: 
            10.18632/oncotarget.17834. eCollection 2017 Sep 8.
  PUBMED    28969075
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.17834"; journalName: "Oncotarget"; date: 
            "2017-05-12- 12"; volume: "8"; issue: "38"; pages: "64344-64357"
COMMENT     Sequence Label: pSIREN-RetroQ-shN
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(55..303)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(113..132)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(283..303)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(327..743)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(343..359)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(375..397)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 acccggtaga attcacgcgt aaaaaatctc ttgaagttgg tactagcaac gcacggatcc
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
      121 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
      181 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat
      241 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
      301 ctcttcctgc ccagatcttc aattccggcg cctagagaag gagtgagggc tggataaagg
      361 gaggatcgag gcggggtcga acgaggaggt tcaaggggga gagacggggc ggatggagga
      421 agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc
      481 aaggcttccc aggtcacgat gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa
      541 aagaccttga tcttaacctg ggtgatgagg tctcggttaa aggtgccgtc tcgcggccat
      601 ccgacgttaa aggttggcca ttctgcagag cagaaggtaa cccaacgtct cttcttgaca
      661 tctaccgact ggttgtgagc gatccgctcg acatctttcc agtgacctaa ggtcaaactt
      721 aagggagtgg taacagtctg gccctaattt tc
//
