LOCUS       sequence-140347-         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSIREN-RetroQ vector containing shRNA sequence to BPTF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  TITLE     BPTF shRNA Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSIREN-RetroQ-BPTF-sh28
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(66..314)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(124..143)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(294..314)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(354..370)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(386..408)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gaattcacgc gtaaaaaaag cacagagaag accatgatct cttgaatcat ggtcttctct
       61 gtgctggatc ctcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa
      121 gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca
      181 agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa
      241 ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg
      301 ggaaataggc cctcttcctg cccagatctt caattccggc gcctagagaa ggagtgaggg
      361 ctggataaag ggaggatcga ggcggggtcg aacgaggagg ttcaaggggg agagacgggg
      421 cggatggagg aagaggaggc ggaggcttag ggtgtacaaa gggcttgacc cagggagggg
      481 ggtcaaaagc caaggcttcc caggtcacga tgtaggggac ctggtctggg tgtccatgcg
      541 ggccaggtga aaagaccttg atcttaacct gggtgatgag gtc
//
