LOCUS       sequence-140350-        1653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses channelrhodopsin fused to mCherry for monosynaptic circuit
            mapping and optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1653)
  TITLE     Jessell Rabies Virus CVS-N2c DG
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: N2c(dG)-hChR2-mCherry
FEATURES             Location/Qualifiers
     source          1..1653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..936
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             949..1653
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1091..1109)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1359..1378
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aaagccacca tggactatgg cggcgctttg tctgccgtcg gacgcgaact tttgttcgtt
       61 actaatcctg tggtggtgaa cgggtccgtc ctggtccctg aggatcaatg ttactgtgcc
      121 ggatggattg aatctcgcgg cacgaacggc gctcagaccg cgtcaaatgt cctgcagtgg
      181 cttgcagcag gattcagcat tttgctgctg atgttctatg cctaccaaac ctggaaatct
      241 acatgcggct gggaggagat ctatgtgtgc gccattgaaa tggttaaggt gattctcgag
      301 ttcttttttg agtttaagaa tccctctatg ctctaccttg ccacaggaca ccgggtgcag
      361 tggctgcgct atgcagagtg gctgctcact tgtcctgtca tccttatccg cctgagcaac
      421 ctcaccggcc tgagcaacga ctacagcagg agaaccatgg gactccttgt ctcagacatc
      481 gggactatcg tgtggggggc taccagcgcc atggcaaccg gctatgttaa agtcatcttc
      541 ttttgtcttg gattgtgcta tggcgcgaac acattttttc acgccgccaa agcatatatc
      601 gagggttatc atactgtgcc aaagggtcgg tgccgccagg tcgtgaccgg catggcatgg
      661 ctgtttttcg tgagctgggg tatgttccca attctcttca ttttggggcc cgaaggtttt
      721 ggcgtcctga gcgtctatgg ctccaccgta ggtcacacga ttattgatct gatgagtaaa
      781 aattgttggg ggttgttggg acactacctg cgcgtcctga tccacgagca catattgatt
      841 cacggagata tccgcaaaac caccaaactg aacatcggcg gaacggagat cgaggtcgag
      901 actctcgtcg aagacgaagc cgaggccgga gccgtgccag cggccgccgt gagcaagggc
      961 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1021 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1081 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1141 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1201 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1261 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1321 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1381 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     1441 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     1501 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     1561 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1621 cactccaccg gcggcatgga cgagctgtac aag
//
