LOCUS       sequence-140352-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized FnCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY004 (pcDNA3.1-hFnCpf1)
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 gccaccatga gcatctacca ggagttcgtc aacaagtatt cactgagtaa gacactgcgg
      181 ttcgagctga tcccacaggg caagacactg gagaacatca aggcccgagg cctgattctg
      241 gacgatgaga agcgggcaaa agactataag aaagccaagc agatcattga taaataccac
      301 cagttcttta tcgaggaaat tctgagctcc gtgtgcatca gtgaggatct gctgcagaat
      361 tactcagacg tgtacttcaa gctgaagaag agcgacgatg acaacctgca gaaggacttc
      421 aagtccgcca aggacaccat caagaaacag attagcgagt acatcaagga ctccgaaaag
      481 tttaaaaatc tgttcaacca gaatctgatc gatgctaaga aaggccagga gtccgacctg
      541 atcctgtggc tgaaacagtc taaggacaat gggattgaac tgttcaaggc taactccgat
      601 atcactgata ttgacgaggc actggaaatc atcaagagct tcaagggatg gaccacatac
      661 tttaaaggct tccacgagaa ccgcaagaac gtgtactcca gcaacgacat tcctacctcc
      721 atcatctacc gaatcgtcga tgacaatctg ccaaagttcc tggagaacaa ggccaaatat
      781 gaatctctga aggacaaagc tcccgaggca attaattacg aacagatcaa gaaagatctg
      841 gctgaggaac tgacattcga tatcgactat aagactagcg aggtgaacca gagggtcttt
      901 tccctggacg aggtgtttga aatcgccaat ttcaacaatt acctgaacca gtccggcatt
      961 actaaattca ataccatcat tggcgggaag tttgtgaacg gggagaatac caagcgcaag
     1021 ggaattaacg aatacatcaa tctgtatagc cagcagatca acgacaaaac tctgaagaaa
     1081 tacaagatgt ctgt
//
