LOCUS       sequence-140353-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized FnCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY004 (pcDNA3.1-hFnCpf1)
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(55..135)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(142..189)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gggccctcta gactcgagcg gccgccactg tgctggatat ctgcagaatt cttaggcata
       61 gtcggggaca tcatatgggt atgcataatc aggcacgtcg tagggataag cgtaatctgg
      121 aacatcgtat gggtaggatc cctttttctt ttttgcctgg ccggcctttt tcgtggccgc
      181 cggccttttg ttatttctat tctggacaaa ctcgaagtat tcctcgttct taatgaccag
      241 gttcagcttc ttcccctcct gattgttctt gatccggccc agcagcatca gtcccttcag
      301 cccgatgtgg taggccccgt tggcgtctgc atcctgaggc atatttttgg gtgcctgtct
      361 gctgtcgaag aagtttccgt tgacatcagc cacggggcta atcagatagt ccagttctgt
      421 cccggtcttt gagttccgca tctgcaggat tgtattcagc actgaggtca gcttggcgaa
      481 aaatttctta tcactctcgc cacagatggc tgccttgatg cattcgccat gtccatactc
      541 gatagagtag tctttcagca gcttttccag ctccttggtt gggtacacct ccctagtgtc
      601 ccaattgtgg tttttatcgg aatttcgaaa gttgatcagg cgagatccga aggaggcaat
      661 ggtccatttc cccttagcgg ccttgtcgcc gaagttcttg taatcgaagg aaaactcgaa
      721 gtaccccttg tccagattat aacagatctt atcgaacttg ctgaaaaatt cctgggactt
      781 gctcactgac tcatatttag ggtacagctg gttgacaaag ccggtcacgg ggcagatctt
      841 ggaagtgaat ccggctggca catagtagat gatgcctgtc tgttttccca ttttcttgaa
      901 agtttcaaag ggagctgtca gctggtaggc tctcaggacg cctccggtct tgtcgaactc
      961 gttatcttta aacaccaggt aattcagctt ttcgatcagc attttctcca gcttctgata
     1021 ga
//
