LOCUS       sequence-140363-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tRNA synthetase/tRNA pair for the in vivo incorporation of several 
            non-standard amino acids, into proteins in E coli in response to the
            amber codon, TAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW,
            Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll 
            D, Rinehart J, Isaacs FJ
  TITLE     Evolution of translation machinery in recoded bacteria enables 
            multi-site incorporation of nonstandard amino acids.
  JOURNAL   Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. 
            Epub 2015 Nov 16.
  PUBMED    26571098
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3372"; 
            journalName: "Nat Biotechnol"; date: "2015-12"; volume: "33"; issue:
            "12"; pages: "1272-1279"
COMMENT     Sequence Label: pEvol-pAcFRS.2.t1
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catcaatcat ccccataatc cttgttagat tatcaatttt aaaaaactaa cagttgtcag
       61 cctgtcccgc tttaatatca tacgccgtta tacgttgttt acgctttgag gaatcccata
      121 tggacgaatt tgaaatgata aagagaaaca catctgaaat tatcagcgag gaagagttaa
      181 gagaggtttt aaaaaaagat gaaaaatctg ctctgatagg ttttgaacca agtggtaaaa
      241 tacatttagg gcattatctc caaataaaaa agatgattga tttacaaaat gctggatttg
      301 atataattat agttttggct gatttacacg cctatttaaa ccagaaagga gagttggatg
      361 agattagaaa aataggagat tataacaaaa aagtttttga agcaatgggg ttaaaggcaa
      421 aatatgttta tggaagtgaa ttccagcttg ataaggatta tacactgaat gtctatagat
      481 tggctttaaa aactacctta aaaagagcaa gaaggagtat ggaacttata gcaagagagg
      541 atgaaaatcc aaaggttgct gaagttatct atccaataat gcaggttaat ggttgtcatt
      601 ataggggcgt tgatgttgat gttggaggga tggagcagag aaaaatacac atgttagcaa
      661 gggagctttt accaaaaaag gttgtttgta ttcacaaccc tgtcttaacg ggtttggatg
      721 gagaaggaaa gatgagttct tcaaaaggga attttatagc tgttgatgac tctccagaag
      781 agattagggc taagataaag aaagcatact gcccagctgg agttgttgaa ggaaatccaa
      841 taatggagat agctaaatac ttccttgaat atcc
//
