LOCUS       sequence-140364-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single-wavelength extracellular glutamate sensor constructed from E.
            coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this 
            sensor is available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Marvin JS, Borghuis BG, Tian L, Cichon J, Harnett MT, Akerboom J, 
            Gordus A, Renninger SL, Chen TW, Bargmann CI, Orger MB, Schreiter 
            ER, Demb JB, Gan WB, Hires SA, Looger LL
  TITLE     An optimized fluorescent probe for visualizing glutamate 
            neurotransmission.
  JOURNAL   Nat Methods. 2013 Feb;10(2):162-70. doi: 10.1038/nmeth.2333. Epub 
            2013 Jan 13.
  PUBMED    23314171
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2333"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "162-70"
COMMENT     Sequence Label: pCMV(MinDis).iGluSnFR
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     125..184
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
ORIGIN
        1 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttggt
       61 accgaactcg gatccactag taacggccgc cagtgtgctg gaattcggct tggggatatc
      121 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
      181 tggtgacaga tctgccgcag gcagcacgct ggacaaaatc gccaaaaacg gtgtgattgt
      241 cgtcggtcac cgtgaatctt cagtgccttt ctcttattac gacaatcagc aaaaagtggt
      301 gggttactcg caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa
      361 accggacttg caggtaaaac tgattccgat tacctcacaa aaccgtattc cactgctgca
      421 aaacggcact ttcgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca
      481 ggcggctttc tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggtgg
      541 cgatatcaaa gattttgcca acctgaaaga caaagccgta gtcgtcactt ccggcactac
      601 ctctgaagtt ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag
      661 cgccaaagat cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat
      721 gatggatgac gtgctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga
      781 aatcgtcggc aagccgcagt ctcaggaggc ctacggttgt atgttgcgta a
//
