LOCUS       sequence-140365-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single-wavelength extracellular glutamate sensor constructed from E.
            coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this 
            sensor is available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Marvin JS, Borghuis BG, Tian L, Cichon J, Harnett MT, Akerboom J, 
            Gordus A, Renninger SL, Chen TW, Bargmann CI, Orger MB, Schreiter 
            ER, Demb JB, Gan WB, Hires SA, Looger LL
  TITLE     An optimized fluorescent probe for visualizing glutamate 
            neurotransmission.
  JOURNAL   Nat Methods. 2013 Feb;10(2):162-70. doi: 10.1038/nmeth.2333. Epub 
            2013 Jan 13.
  PUBMED    23314171
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2333"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "162-70"
COMMENT     Sequence Label: pCMV(MinDis).iGluSnFR
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..161)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     367..386
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     626..647
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(753..774)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcatgatga ggatgataag ggagatgatg gtgagcacca ccagggccag gatggctgag
       61 atcaccacca ccttaaaggg caaggagtgt ggcaccacga tgacctcctg cgtgtcctgg
      121 cccacagcat tcagatcctc ttctgagatg agtttttgtt cgtcgacctg cagtttcagt
      181 gccttgtcat tcggttcttt gaacagtgct ttcatttcgt ctgacagttc gaaattcatg
      241 ttcagggggt tgttaaagtt gtactccagc ttatgcccca ggatgttgcc gtcctccttg
      301 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      361 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gatgtagcct
      421 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
      481 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      541 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc accctcgccg
      601 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      661 ccggtgaaca gctcctcgcc cttgctcacc atgctccctc cggtaccgcc cttgtacagc
      721 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      781 cgcttctcgt tgg
//
