LOCUS       sequence-140367-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tRNA synthetase/tRNA pair for the in vivo incorporation of several 
            non-standard amino acids, into proteins in E coli in response to the
            amber codon, TAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW,
            Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll 
            D, Rinehart J, Isaacs FJ
  TITLE     Evolution of translation machinery in recoded bacteria enables 
            multi-site incorporation of nonstandard amino acids.
  JOURNAL   Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. 
            Epub 2015 Nov 16.
  PUBMED    26571098
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3372"; 
            journalName: "Nat Biotechnol"; date: "2015-12"; volume: "33"; issue:
            "12"; pages: "1272-1279"
COMMENT     Sequence Label: pEvol-pAcFRS.1.t1
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caatcatccc cataatcctt gttagattat caattttaaa aaactaacag ttgtcagcct
       61 gtcccgcttt aatatcatac gccgttatac gttgtttacg ctttgaggaa tcccatatgg
      121 acgaatttga aatgataaag agaaacacat ctgaaattat cagcgaggaa gagttaagag
      181 aggttttaaa aaaagatgaa aaatctgctc tgataggttt tgaaccaagt ggtaaaatac
      241 atttagggca ttatctccaa ataaaaaaga tgattgattt acaaaatgct ggatttgata
      301 taattatatt gttggctgat ttacacgcct atttaaacca gaaaggagag ttggatgaga
      361 ttagaaaaat aggagattat aacaaaaaag tttttgaagc aatggggtta aaggcaaaat
      421 atgtttatgg aagtgaattc cagcttgata aggattatac actgaatgtc tatagattgg
      481 ctttaaaaac taccttaaaa agagcaagaa ggagtatgga acttatagca agagaggatg
      541 aaaatccaaa ggttgctgaa gttatctatc caataatgca ggttaatggt tgtcattata
      601 ggggcgttga tgttgatgtt ggagggatgg agcagagaaa aatacacatg ttagcaaggg
      661 agcttttacc aaaaaaggtt gtttgtattc acaaccctgt cttaacgggt ttggatggag
      721 aaggaaagat gagttcttca aaagggaatt ttatagctgt tgatgactct ccagaagaga
      781 ttagggctaa gataaagaaa gcatactgcc cagctggagt tgttgaagga aatccaataa
      841 tggagatagc taaatacttc cttgaatatc cttta
//
