LOCUS       sequence-140372-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tRNA synthetase/tRNA pair for the in vivo incorporation of several 
            non-standard amino acids, into proteins in E coli in response to the
            amber codon, TAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW,
            Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll 
            D, Rinehart J, Isaacs FJ
  TITLE     Evolution of translation machinery in recoded bacteria enables 
            multi-site incorporation of nonstandard amino acids.
  JOURNAL   Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. 
            Epub 2015 Nov 16.
  PUBMED    26571098
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3372"; 
            journalName: "Nat Biotechnol"; date: "2015-12"; volume: "33"; issue:
            "12"; pages: "1272-1279"
COMMENT     Sequence Label: pEvol-pAzFRS.2.t1
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcaatcatc cccataatcc ttgttagatt atcaatttta aaaaactaac agttgtcagc
       61 ctgtcccgct ttaatatcat acgccgttat acgttgttta cgctttgagg aatcccatat
      121 ggacgaattt gaaatgataa agagaaacac atctgaaatt atcagcgagg aagagttaag
      181 agaggtttta aaaaaggatg aaaaatctgc tctgataggt tttgaaccaa gtggtaaaat
      241 acatttaggg cattatctcc aaataaaaaa gatgattgat ttacaaaatg ctggatttga
      301 tataattata ttgttggctg atttacacgc ctatttaaac cagaaaggag agttggatga
      361 gattagaaaa ataggagatt ataacaaaaa agtttttgaa gcaatggggt taaaggcaaa
      421 atatgtttat ggaagtactt atatgcttga taaggattat acactgaatg tctatagatt
      481 ggctttaaaa actaccttaa aaagggcaag aaggagtatg gaacttatag caagagagga
      541 tgaaaatcca aaggttgctg aagttatcta tccaataatg caggttaatg gttgtcatta
      601 taggggcgtt gatgttgctg ttggagggat ggagcagaga aaaatacaca tgttagcaag
      661 ggagctttta ccaaaaaagg ttgtttgtat tcacaaccct gtcttaacgg gtttggatgg
      721 agaaggaaag atgagttctt caaaagggaa ttttatagct gttgatgact ctccagaaga
      781 gattagggct aagataaaga aagcatactg cccagctgga gttgttgaag gaaatccaat
      841 aatggagata gctaaatact tccttgaata tccttta
//
