LOCUS       sequence-140386-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing of iBlueberry and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Yu D, Dong Z, Gustafson WC, Ruiz-Gonzalez R, Signor L, Marzocca F, 
            Borel F, Klassen MP, Makhijani K, Royant A, Jan YN, Weiss WA, Guo S,
            Shu X
  TITLE     Rational design of a monomeric and photostable far-red fluorescent 
            protein for fluorescence imaging in vivo.
  JOURNAL   Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015
            Dec 31.
  PUBMED    26549191
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2843"; 
            journalName: "Protein Sci"; date: "2016-02"; volume: "25"; issue: 
            "2"; pages: "308-15"
COMMENT     Sequence Label: iBlueberry T2A mCherry
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(281..298)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    287..511
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      557..936
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(644..663)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     854..875
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
       61 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      121 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      181 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      241 tacaagtaat ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
      301 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
      361 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
      421 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
      481 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
      541 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      601 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      661 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      721 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      781 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      841 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
      901 tattcttttg atttataagg gattttgccg atttcg
//
