LOCUS       sequence-140388-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Myr-mIFP in Zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Yu D, Dong Z, Gustafson WC, Ruiz-Gonzalez R, Signor L, Marzocca F, 
            Borel F, Klassen MP, Makhijani K, Royant A, Jan YN, Weiss WA, Guo S,
            Shu X
  TITLE     Rational design of a monomeric and photostable far-red fluorescent 
            protein for fluorescence imaging in vivo.
  JOURNAL   Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015
            Dec 31.
  PUBMED    26549191
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2843"; 
            journalName: "Protein Sci"; date: "2016-02"; volume: "25"; issue: 
            "2"; pages: "308-15"
COMMENT     Sequence Label: pCS2+_Myr-mIFP
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..152)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(202..220)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(203..220)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ttgtccggct ctttcacagc cagcaggata ccgtgcggct gaatggagcc cgccaggtgg
       61 atctgctcgc gttcacagtt agccagaaac tcgtggccgt atgctgaggt agtcagcggt
      121 accgacttgg gcttgctctt gctgctcccc atggtggcaa cgaatcgatg ggatcctgca
      181 aaaagaacaa gtagcttgta ttctatagtg tcacctaaat caagcttgct ccgacgtccc
      241 caggcagaat ggcggttccc taaacgagca ttgcttatat agacctccca ttaggcacgc
      301 ctaccgccca tttacgtcaa tggaacgccc atttgcgtca ttgcccctcc ccattgacgt
      361 caatggggat gtacttggca gccatcgcgg gccatttacc gccattgacg tcaatgggag
      421 tactgccaat gtaccctggc gtacttccaa tagtaatgta cttgccaagt tactattaat
      481 agatattgat gtactgccaa gtgggccatt taccgtcatt gacgtcaata gggggcgtga
      541 gaacggatat gaatgggcaa tgagccatcc cattgacgtc aatggtgggt ggtcctattg
      601 acgtcaatgg gcattgagcc aggcgggcca tttaccgtaa ttgacgtcaa tgggggaggc
      661 gccatatacg tcaataggac cgcccatatg acgtcaatag gaaagaccat atatagagac
      721 cattgacgtc aatgggggag tggctatggg cggtattagg aagccccata tatggtatat
      781 gggaccgccc attgggaggg gctatctacg tcaataggaa aacccatata tggaaatact
      841 atatggcata gggccaatac atagtattga acctggccaa tagccatatt ggcatagggc
      901 catattggat attgcctata tattgatcct ggcatatagc ca
//
