LOCUS       sequence-140397-        3131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of SERCA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3131)
  TITLE     Nakamoto lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1/HisA/Serca1
FEATURES             Location/Qualifiers
     source          1..3131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..44
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             45..74
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             96..116
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggncgnnnnt gtcgtactac gattacaagg atgacgacga taagcatcac catcaccatc
       61 accatcacca tcacgattac gatatcccaa cgaccgaaaa cctgtatttt cagggcgcca
      121 tggaggccgc gcactccaag agcacagagg aatgtctggc ctattttggg gtgagcgaga
      181 ccacaggcct caccccggac caagtgaagc gacatctaga gaaatacggc cacaatgagc
      241 ttcctgctga ggaagggaaa tccctgtggg agctggtgat agagcagttt gaagacctcc
      301 tggtgcggat tcttctgctg gccgcctgca tctcctttgt gctggcctgg tttgaagaag
      361 gggaagagac catcactgcc ttcgttgagc cctttgtcat cctcctgatc ctcattgcca
      421 atgccatcgt gggagtttgg caggagcgga acgctgagaa cgccatagag gcgctgaagg
      481 aatatgagcc cgagatgggg aaggtgtacc gggctgaccg caagtcagtg caaaggatca
      541 aggctcggga catcgtcccc ggggacatcg tggaggtggc ggttggggac aaagtccctg
      601 cagacatccg catcctgtct atcaagtcca ccaccctccg cgtggaccag tccatcctga
      661 caggcgagtc cgtgtccgtc atcaagcaca cggagccagt ccctgacccg cgggctgtca
      721 accaggacaa gaagaacatg cttttctcgg gtaccaacat cgcggccggc aaggccctgg
      781 gcatcgtggc caccaccggc gtgagcaccg agatcgggaa gatccgtgac cagatggccg
      841 ccacggagca ggacaagacg ccgctgcagc agaagctgga tgagttcggg gagcagctgt
      901 ccaaggtcat ctccctcatc tgcgtggccg tgtggcttat caacatcggc cacttcaacg
      961 accccgtcca cgggggctcc tggatccgcg gtgccatcta ctacttcaag atcgccgtgg
     1021 ccttggctgt ggctgcgatc ccagaaggtc ttcccgctgt catcactacc tgcctggccc
     1081 tgggcacccg ccggatggcg aagaagaacg ccatcgtgag gagcctgccc tctgtggaga
     1141 ccctgggctg cacctctgtc atctgctctg acaagactgg caccctcacc accaaccaga
     1201 tgtctgtgtg caagatgttc atcatcgaca aggtggacgg agacttctgt tcgctgaacg
     1261 agttctccat caccggctcc acctacgctc cagaggggga ggtcctgaag aatgataaac
     1321 ccatccggtc agggcagttt gatgggctgg tggagctggc caccatttgt gccctgtgca
     1381 atgattcctc cttggacttc aatgagacca aaggcgtcta tgagaaggtg ggtgaggcca
     1441 cggagacggc gctcaccacc ctggtggaga agatgaatgt gttcaacacg gaagttcgga
     1501 acctctcgaa ggtggagaga gccaacgcct gcaactcggt gatccgccag ctcatgaaga
     1561 aagagttcac cctggagttc tcccgagaca ggaagtccat gtctgtgtac tgttctccag
     1621 ccaaatcttc ccgcgctgct gtgggcaaca agatgtttgt caagggcgcc cccgaggggg
     1681 tcatcgaccg ctgtaactac gtgcgagtcg gcaccacccg ggtgcccatg actgggccgg
     1741 tgaaggagaa gatcctctca gtgatcaagg agtggggcac cggccgggac acgctgcgct
     1801 gcctggccct ggccacccgc gacacgccgc ccaagcgaga ggaaatggtg ctggacgact
     1861 cctcccggtt catggagtac gagacggacc tgacgttcgt gggcgtcgtg ggcatgctgg
     1921 acccgccccg caaggaggtc atgggttcca tccagctgtg ccgggacgcc gggatccgtg
     1981 tcatcatgat caccggcgac aacaagggca cggccatcgc catctgccgc cgcatcggca
     2041 tctttgggga gaacgaggag gtggcagacc gcgcctacac cggccgcgag tttgacgacc
     2101 tgcccctggc cgagcagcgg gaagcctgcc gccgcgcctg ctgcttcgcg cgcgtggaac
     2161 cctcccacaa gtccaagatc gtggaatacc tgcagtccta cgatgagatc acggccatga
     2221 caggggatgg cgtcaacgac gcccctgccc tgaagaaggc cgagatcggc atagctatgg
     2281 gatctggcac cgccgtggcc aagacagcgt ctgagatggt gctggcggac gacaacttct
     2341 ccaccatcgt ggccgccgtg gaggagggcc gcgccatcta caacaacatg aagcagttca
     2401 tccgctacct catctcctcc aacgtgggcg aggtggtctg catcttcctg acggccgcct
     2461 tggggctgcc cgaggccctg atccctgtgc agctgctgtg ggtgaacctg gtgacggacg
     2521 ggctcccggc cacagccctg ggcttcaacc caccagacct ggacatcatg gaccggcccc
     2581 cccggagtcc caaggagccc ctgatcagtg gctggctctt cttccgctac atggccatcg
     2641 ggggctatgt gggtgcagcc accgtgggag ccgctgcctg gtggttcatg tatgcggagg
     2701 atgggccggg tgtcacctac caccagctga cccacttcat gcagtgcacc gaggaccacc
     2761 ctcactttga gggtctggac tgtgagatct ttgaggcccc agagcccatg actatggcct
     2821 tgtctgtgct ggtgaccatc gagatgtgca atgctctcaa cagcctgtcc gagaaccagt
     2881 ccttgatgcg gatgccgccc tgggtgaaca tctggctgct gggctccatc tgcctgtcca
     2941 tgtccctcca cttcctcatc ctctacgtcg accccctgcc gatgatcttc aagctcaagg
     3001 ccctggacct cacccagtgg ctcatggtcc tcaagatctc acttccagtc atcgggctgg
     3061 atgaaatact caagttcatt gctcggaact acctggagga tccagaagat gaaaggagga
     3121 agtaactcga g
//
