LOCUS       sequence-140400-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pMA7CR_2.0 contains arabinose inducible lambda/RED beta-protein and 
            dam, and aTc inducible CRISPR/Cas9 and recX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Ronda C, Pedersen LE, Sommer MO, Nielsen AT
  TITLE     CRMAGE: CRISPR Optimized MAGE Recombineering.
  JOURNAL   Sci Rep. 2016 Jan 22;6:19452. doi: 10.1038/srep19452.
  PUBMED    26797514
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-01-22"; volume: "6:19452. doi"; pages: " 
            10.1038/srep19452"
COMMENT     Sequence Label: pMA7CR_2.0
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..840
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MGTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
ORIGIN
        1 actctctact gtttctccat acccgttttt ttgggctagc aggaggaatt caccatgggt
       61 actgcactcg caacgctggc tgggaagctg gctgaacgtg tcggcatgga ttctgtcgac
      121 ccacaggaac tgatcaccac tcttcgccag acggcattta aaggtgatgc cagcgatgcg
      181 cagttcatcg cattactgat cgttgccaac cagtacggcc ttaatccgtg gacgaaagaa
      241 atttacgcct ttcctgataa gcagaatggc atcgttccgg tggtgggcgt tgatggctgg
      301 tcccgcatca tcaatgaaaa ccagcagttt gatggcatgg actttgagca ggacaatgaa
      361 tcctgtacat gccggattta ccgcaaggac cgtaatcatc cgatctgcgt taccgaatgg
      421 atggatgaat gccgccgcga accattcaaa actcgcgaag gcagagaaat cacggggccg
      481 tggcagtcgc atcccaaacg gatgttacgt cataaagcca tgattcagtg tgcccgtctg
      541 gccttcggat ttgctggtat ctatgacaag gatgaagccg agcgcattgt cgaaaatact
      601 gcatacactg cagaacgtca gccggaacgc gacatcactc cggttaacga tgaaaccatg
      661 caggagatta acactctgct gatcgccctg gataaaacat gggatgacga cttattgccg
      721 ctctgttccc agatatttcg ccgcgacatt cgtgcatcgt cagaactgac acaggccgaa
      781 gcagtaaaag ctcttggatt cctgaaacag aaagccgcag agcagaaggt ggcagcatga
      841 agtcggtgtg gagtgaaacg atgaagaaaa a
//
