LOCUS       sequence-140402-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HDR template to generate Cdk2-T160E-siR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Kaulich M, Lee YJ, Lonn P, Springer AD, Meade BR, Dowdy SF
  TITLE     Efficient CRISPR-rAAV engineering of endogenous genes to study 
            protein function by allele-specific RNAi.
  JOURNAL   Nucleic Acids Res. 2015 Apr 20;43(7):e45. doi: 10.1093/nar/gku1403. 
            Epub 2015 Jan 13.
  PUBMED    25586224
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-04-20"; volume: "43(7):e45. doi"; pages: " 
            10.1093/nar/gku1403. Epub 2015 Jan 13"
COMMENT     Sequence Label: pAAV-SEPT_Cdk2-T160E-siR
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(18..51)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gccgggatga gttgggaata acttcgtata gcatacatta tacgaagtta tccaaatttt
       61 aaggtaccac tgtgcatatg ccctagggtt ggactgaaca tcaaagttga aactctagtg
      121 agtcaaccta gcaactcagg tgggaggtca gatgaaactc agataaacgg gatttgagag
      181 cacttggtaa attcctccaa aaagcccttc catttggtgg aagacctagc tagtgagtcc
      241 ctattgtcta ttttagggct ggattcttca ctcccagagc tactttcaat ctattaacaa
      301 acattttttc aatgcacagg atgtagaaaa gggatggaaa attgagtaag acttggtcct
      361 tatcctctct gggctgacag tccattggga gaaatagctt gtaaatatgt aactataatc
      421 caacataata aaggctttag tagagtttta ggggcacaga gcaaacccag tctgctcact
      481 gtaatggaga aacacagtcc tctctttctc ctttgtcaga gctatctgtt ccagctgctc
      541 cagggcctag ctttctgcca ttctcatcgg gtcctccacc gagaccttaa acctcagaat
      601 ctgcttatta acacagaggg ggccatcaag ctagcagact ttggactagc cagagctttt
      661 ggagtccctg ttcgtactta cgagcatgag gtgagtccct ttatgtcttt tttctctgag
      721 cttcccaaga ggtgttaact agggtattca caaagttact aaaaatatct ggctaacagt
      781 ttcttactag gtagaaataa tctcttgaca tcctaaagag tcttagggta tgcatggaat
      841 tcatactgtg ttgctaactg ggcccacacc tgtaatacca atactttggg aggctgaggt
      901 gggaggatca cttgagccca ggagttcgag accatcatgg gcaacatagc gagaccc
//
