LOCUS       sequence-140406-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HDR template to generate Cdk2-T160A-siR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Kaulich M, Lee YJ, Lonn P, Springer AD, Meade BR, Dowdy SF
  TITLE     Efficient CRISPR-rAAV engineering of endogenous genes to study 
            protein function by allele-specific RNAi.
  JOURNAL   Nucleic Acids Res. 2015 Apr 20;43(7):e45. doi: 10.1093/nar/gku1403. 
            Epub 2015 Jan 13.
  PUBMED    25586224
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-04-20"; volume: "43(7):e45. doi"; pages: " 
            10.1093/nar/gku1403. Epub 2015 Jan 13"
COMMENT     Sequence Label: pAAV-SEPT_Cdk2-T160A-siR
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(10..43)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gagttgggaa taacttcgta tagcatacat tatacgaagt tatccaaatt ttaaggtacc
       61 actgtgcata tgtatgaatt cgatatcaag cttatcgatc cctagggttg gactgaacaa
      121 tcaaagttga aactctagtg agtcaaccta gcaactcagg tgggaggtca gatgaaactc
      181 agataaacgg gatttgagag cacttggtaa attcctccaa aaagcccttc catttggtgg
      241 aagacctagc tagtgagtcc ctattgtcta ttttagggct ggattcttca ctcccagagc
      301 tactttcaat ctattaacaa acattttttc aatgcacagg atgtagaaaa gggatggaaa
      361 attgagtaag acttggtcct tatcctctct gggctgacag tccattggga gaaatagctt
      421 gtaaatatgt aactataatc caacataata aaggctttag tagagtttta ggggcacaga
      481 gcaaacccag tctgctcact gtaatggaga aacacagtcc tctctttctc ctttgtcaga
      541 gctatctgtt ccagctgctc cagggcctag ctttctgcca ttctcatcgg gtcctccacc
      601 gagaccttaa acctcagaat ctgcttatta acacagaggg ggccatcaag ctagcagact
      661 ttggactagc cagagctttt ggagttcctg ttcgtactta cgcccatgag gtgagtccct
      721 ttatgtcttt tttctctgag cttcccaaga ggtgttaact agggtattca caaagttact
      781 aaaaatatct ggctaacagt ttcttactag gtagaaataa tctcttgaca tcctaaagag
      841 tcttagggta tgcatg
//
