LOCUS       sequence-140410-         348 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RNF169 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Panier S, Ichijima Y, Fradet-Turcotte A, Leung CC, Kaustov L, 
            Arrowsmith CH, Durocher D
  TITLE     Tandem protein interaction modules organize the ubiquitin-dependent 
            response to DNA double-strand breaks.
  JOURNAL   Mol Cell. 2012 Aug 10;47(3):383-95. doi: 
            10.1016/j.molcel.2012.05.045. Epub 2012 Jun 27.
  PUBMED    22742833
REFERENCE   2  (bases 1 to 348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.05.045"; journalName: "Mol Cell"; date: 
            "2012-08-10- 10"; volume: "47"; issue: "3"; pages: "383-95"
COMMENT     Sequence Label: pcDNA5-FRT/TO-Flag-RNF169
FEATURES             Location/Qualifiers
     source          1..348
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..20
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     30..54
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             154..177
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
       61 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      121 actctagcgt ttaaacttaa gcttggtacc atggactaca aagacgatga cgataaagcc
      181 gccagtttaa acggcgcgcc attaattaaa atggcggctg caggtccgag tactcgggcc
      241 tcttccgcgg cggcagcagc cgctctgagt cggcggggcc ggcggggccg ctgtgacgag
      301 acggcggcag ctaagactgg ggccccaggc ccggcttctg gaccttcg
//
