LOCUS       sequence-140412-        1490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of mitoLbNOX 
            which is a water-forming NADH oxidase that can be used to increase 
            NAD+/NADH ratio in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Titov DV, Cracan V, Goodman RP, Peng J, Grabarek Z, Mootha VK
  TITLE     Complementation of mitochondrial electron transport chain by 
            manipulation of the NAD+/NADH ratio.
  JOURNAL   Science. 2016 Apr 8;352(6282):231-5. doi: 10.1126/science.aad4017. 
            Epub 2016 Apr 7.
  PUBMED    27124460
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad4017"; journalName: "Science"; date: "2016-04-8-
            8"; volume: "352"; issue: "6282"; pages: "231-5"
COMMENT     Sequence Label: pUC57-mitoLbNOX
FEATURES             Location/Qualifiers
     source          1..1490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1458..1481
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcggccgcat gctcgctaca agggtcttta gcctcgtcgg aaagagagct atcagcacct
       61 ccgtctgcgt gagagctcat aaggtcaccg tggtcggatg cacccatgcc ggcaccttcg
      121 ccatcaagca aatcctcgct gagcaccctg acgccgaggt caccgtctac gagaggaacg
      181 atgtgatctc cttcctgtcc tgtggcatcg ccctctacct gggcggaaaa gtggccgatc
      241 cccaaggcct cttctacagc tcccctgaag aactgcagaa gctgggcgct aatgtgcaga
      301 tgaaccacaa cgtgctggcc atcgaccctg accaaaagac cgtcacagtc gaggacctca
      361 ccaatcacgc ccagaccacc gagtcctacg acaaactggt gatgacctcc ggaagctggc
      421 ctatcgtgcc caaaatcccc ggcatcgaca gcgatagggt gaagctctgc aagaattggg
      481 cccacgccca ggctctgatt gaggacgcca aggaggccaa gaggatcacc gtcatcggcg
      541 ccggatacat cggagccgaa ctggccgagg cctactccac aacaggccac gacgtcaccc
      601 tgattgacgc catggctagg gtcatgccca agtacttcga tgccgacttc accgacgtca
      661 tcgaacagga ctacagggac catggcgtgc aactcgctct gggcgagaca gtggagagct
      721 tcaccgacag cgccaccggc ctcacaatca agacagacaa gaactcctat gagaccgacc
      781 tggccatcct ctgcattggc tttaggccca acacagacct gctgaaaggc aaagtggaca
      841 tggcccctaa cggcgccatc attaccgacg actacatgag gtccagcaac cctgatattt
      901 tcgctgctgg cgactccgcc gccgtccatt acaaccccac acaccaaaac gcctacattc
      961 ccctcgctac caacgccgtc aggcagggaa tcctcgtcgg aaagaacctc gtcaagccca
     1021 cagtgaagta catgggaacc cagtccagct ccggactggc cctctatgac aggacaattg
     1081 tctccacagg cctcacactg gccgccgcca agcaacaagg cctcaatgcc gagcaggtca
     1141 tcgtggagga caactatagg cccgagttca tgccttccac cgagcccgtc ctcatgagcc
     1201 tggtcttcga ccccgataca cacagaatcc tgggaggcgc cctgatgtcc aaatacgacg
     1261 tgtcccagag cgctaatacc ctgtccgtct gcatccagaa cgagaacacc atcgatgacc
     1321 tggccatggt ggacatgctg ttccagccca atttcgacag gcccttcaac tacctgaaca
     1381 ttctcgccca ggctgcccaa gctaaagtgg cccaatccgt caacgctggt ggatctggtg
     1441 gatctggtgg atctatggat tacaaggatg acgatgacaa gtaaacgcgt
//
