LOCUS       sequence-140415-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multi-purpose rapid titer bacmam expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Morales-Perez CL, Noviello CM, Hibbs RE
  TITLE     Manipulation of Subunit Stoichiometry in Heteromeric Membrane 
            Proteins.
  JOURNAL   Structure. 2016 Mar 24. pii: S0969-2126(16)00082-4. doi: 
            10.1016/j.str.2016.03.004.
  PUBMED    27041595
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2016 Mar 24. pii: S0969-2126(16)00082-4. doi: 
            10.1016/j.str.2016.03.004."
COMMENT     Sequence Label: pEZT-BM
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(88..106)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(149..168)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          151..283
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(398..422)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(419..622)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(448..468)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        623..926
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt aagcttggta cctcggatcc
       61 ctgcagtcgc ggccgcctcg aggctagcct atagtgagtc gtattaagta ctctagcctt
      121 aagagctgta attgaactgg gagtggacac ctgtggagag aaaggcaaag tggatgtcag
      181 taagaccaat aggtgcctat cagaaacgca agagtcttct ctgtctcgac aagcccagtt
      241 tctattggtc tccttaaacc tgtcttgtaa ccttgatact tacctgccca gtgcctcacg
      301 accaacttct gcagcttaag ttcgagactg ttgtgtcaga agcactgact gcgttagcaa
      361 tttaactgtg ataaactacc gcaataaagc ttctagtgat ctgacggttc actaaacgag
      421 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aacggggcgg
      481 ggttattacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      541 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattgg
      601 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      661 aagtaggaaa gtcccgtaag gtcatgtact gggcataatg ccaggcgggc catttaccgt
      721 cattgacgtc aatagggggc ggacttggca tatgatacac ttgatgtact gccaagtggg
      781 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
      841 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
      901 ggccatttac cgtaagttat gtaacgcgga actcc
//
