LOCUS       sequence-140416-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vector bearing an NheI restriction site for the two-step cloning 
            method in Wang and Szaro, Dev Biol, 2015.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Wang C, Szaro BG
  TITLE     A method for using direct injection of plasmid DNA to study 
            cis-regulatory element activity in F0 Xenopus embryos and tadpoles.
  JOURNAL   Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010.
            Epub 2014 Nov 20.
  PUBMED    25448690
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2014.11.010"; journalName: "Dev Biol"; date: 
            "2015-02-1- 1"; volume: "398"; issue: "1"; pages: "11-23"
COMMENT     Sequence Label: pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..110
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     intron          242..814
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca gaagtcagat
       61 gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga tctcagtggt
      121 atttgtgagc cagggcattg gccacaccag ccaccacctt ctgataggca gcctgcacct
      181 gaggagtgaa ttctttgcca aaatgatgag acagcacaac aaccagcacg ttgcccagga
      241 gctgtaggaa agagaagaag gcatgaacat ggttagcaga ggggcccggt ttggactcag
      301 agtattttat cctcatctca aacagtgtat atcattgtaa ccataaagag aaaggcagga
      361 tgatgaccag ggtgtagttg tttctaccaa taagaatatt tccacgccag ccagaattta
      421 tatgcagaaa tattctacct tatcatttaa ttataacaat tgttctctaa aactgtgctg
      481 aagtacaata taatataccc tgattgcctt gaaaaaaaag tgattagaga aagtacttac
      541 aatctgacaa ataaacaaaa gtgaatttaa aaattcgtta caaatgcaag ctaaagttta
      601 acgaaaaagt tacagaaaat gaaaagaaaa taagaggaga caatggttgt caacagagta
      661 gaaagtgaaa gaaacaaaat tatcatgagg gtccatggtg atacaaggga catcttccca
      721 ttctaaacaa caccctgaaa actttgcccc ctccatataa catgaatttt acaatagcga
      781 aaaagaaaga acaatcaagg gtccccaaac tcaccctgaa gttctcagga tccgcggccg
      841 ctcacttgta cagctcgtcc atgccatgtg tgatcccagc agcggtcaca aactccagca
      901 ggaccatgtg gtctctcttt tcgttgggat ctttagacag ggcagactgg gtggacaggt
      961 aatggtt
//
