LOCUS       sequence-140417-        2132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from 
            Bba_B0030 to adjust spacing in MC system. [B:B0030m:C].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Densmore
  TITLE     B0030m_BC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: B0030m_BC
FEATURES             Location/Qualifiers
     source          1..2132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     53..72
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(291..395)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     463..481
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(485..528)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    531..552
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             578..589
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    606..626
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(632..649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(803..1391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(883..902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1562..2132,1..221,222..290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
       61 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      361 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
      421 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
      481 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
      541 ccgcttctag agactagtgg gtctcatact agagattaaa gaggagaaat actaaatgag
      601 agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg
      661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      781 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
      841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1021 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1081 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1141 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1201 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1261 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1321 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1381 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1441 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1501 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1561 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1621 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1681 ccagtgctgc aatgataccg cgcgacccac gctcaccggc tccagattta tcagcaataa
     1741 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1801 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1861 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1921 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1981 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2041 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2101 ctgtgactgg tgagtactca accaagtcat tc
//
