LOCUS       sequence-140431-        4743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pas2p peroxisomal membrane biogenesis protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK139
FEATURES             Location/Qualifiers
     source          1..4743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             2426..2455
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2471..2488
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2567..2813
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        2828..3239
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        3247..3294
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3313..3687
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      3753..4000
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3769..3787)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(4075..4663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4155..4174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattcac gtggatcatc
      961 attgcaaagc cataaactct gagaggaaca caagtgcata caatcactag atatttgcaa
     1021 agtcgtaatg ttggagtaca cggcaggatt aataagacga aacaagaaaa agtttcttat
     1081 atcctctgga atcataggag taggttacta tgtaaccaaa actatcaata acaagatcca
     1141 agaattccaa aaccgaatta gagaggaaaa ctttgccaaa gagcaaatca agcgtcggtt
     1201 tcaccagacg caaagtgatt gctacatgac ctttctatca ctgctgccag tactttgtga
     1261 accaattatg gacgatttgc cagtagagac tatcaccaag cagctccaaa ttagacggct
     1321 agaaaaacaa attggcaaca aagatgttaa gaattccgga tccactgtgc ttagtgacga
     1381 tttctcaaca tctcaagagg gagcgatatc ggaggatact aacaaacctc ctgaactgaa
     1441 aagtaaaaat caattgtggc aagagctaaa aatcaaagca ataacaagat ttctcacgct
     1501 gatatattgt gaatccttgc tgattgtatt cttacacctt cagcttaata ttctatcgcg
     1561 caagtcttat cttgagactg caatcaggct ggcttctgag acccagggga tagatttggt
     1621 agatcaagag tctaatagtg acttttctgg caacactcag gatgaaaact tatccgaaca
     1681 agcattcttg agttttagtt ggtggctgct caacaaggga tggcttgaga taaagaacaa
     1741 gattgagcca tgtgttgaac aacactttgg aggtataaat cccagacagc aattgaagat
     1801 aaatgagttt gccgagcttt taaacaaatg tcaaaattgt attgacctga aggttttaaa
     1861 tcttaccgaa gaggatatac atttgggagt cggtgttatc gaagaccagt cccagcctgt
     1921 tggaagaaaa agcactaact ttatcacaaa cgctttatta ccacccaaag agtttgagtt
     1981 tttccttttg caacagacaa atgatttaga ctttttatcc cgcttcaaca ataatatcgt
     2041 caatactgag agcttgaaca tgttattgga tgaattaaac aactatctga acaatgcgga
     2101 catcaatctg atagtcaaca aattggctac cctaggtatt accaaagttc tggatgaaat
     2161 agttctcaac ctattacaga agaatcgacc gaacccaatt tcagatatga atatacgtga
     2221 tattgatctc aatgattttc ccccctacaa attagcagct ttgctggcaa acataaccaa
     2281 acagtctatt tcgttgacta acaactctgt ggagaaccct attttagcag acttaaacaa
     2341 tttaccggaa cttaatgatc tcagcgcaag tgtatactct aattttgatc ctcgctcgag
     2401 ccgcggcggc cgccagcttg ggcccgaaca aaaactcatc tcagaagagg atctgaatag
     2461 cgccgtcgac catcatcatc atcatcattg agttttagcc ttagacatga ctgttcctca
     2521 gttcaagttg ggcacttacg agaagaccgg tcttgctaga ttctaatcaa gaggatgtca
     2581 gaatgccatt tgcctgagag atgcaggctt catttttgat acttttttat ttgtaaccta
     2641 tatagtatag gatttttttt gtcattttgt ttcttctcgt acgagcttgc tcctgatcag
     2701 cctatctcgc agctgatgaa tatcttgtgg taggggtttg ggaaaatcat tcgagtttga
     2761 tgtttttctt ggtatttccc actcctcttc agagtacaga agattaagtg agaccttcgt
     2821 ttgtgcggat cccccacaca ccatagcttc aaaatgtttc tactcctttt ttactcttcc
     2881 agattttctc ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact
     2941 aaattttccc tctttcttcc tctagggtgt cgttaattac ccgtactaaa ggtttggaaa
     3001 agaaaaaaga gaccgcctcg tttctttttc ttcgtcgaaa aaggcaataa aaatttttat
     3061 cacgtttctt tttcttgaaa tttttttttt tagttttttt ctctttcagt gacctccatt
     3121 gatatttaag ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc
     3181 tattacaact ttttttactt cttgttcatt agaaagaaag catagcaatc taatctaagg
     3241 ggcggtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     3301 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     3361 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     3421 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     3481 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     3541 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     3601 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     3661 cacttcgtgg ccgaggagca ggactgacac gtccgacggc ggcccacggg tcccaggcct
     3721 cggagatccg tccccctttt cctttgtcga tatcatgtaa ttagttatgt cacgcttaca
     3781 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
     3841 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
     3901 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
     3961 gcttgagaag gttttgggac gctcgaaggc tttaatttgc aagctggaga ccaacatgtg
     4021 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4081 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4141 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4201 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4261 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4321 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4381 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4441 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4501 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4561 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4621 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4681 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4741 atc
//
