LOCUS       sequence-140432-        5856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a 
            fusion to Pas2p, with c-myc tag and His6-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK142
FEATURES             Location/Qualifiers
     source          1..5856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             3539..3568
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3584..3601
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3680..3926
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        3941..4352
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        4360..4407
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4426..4800
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      4866..5113
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4882..4900)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(5188..5776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5268..5287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattcac gtggatcatc
      961 attgcaaagc cataaactct gagaggaaca caagtgcata caatcactag atatttgcaa
     1021 agtcgtaatg ttggagtaca cggcaggatt aataagacga aacaagaaaa agtttcttat
     1081 atcctctgga atcataggag taggttacta tgtaaccaaa actatcaata acaagatcca
     1141 agaattccaa aaccgaatta gagaggaaaa ctttgccaaa gagcaaatca agcgtcggtt
     1201 tcaccagacg caaagtgatt gctacatgac ctttctatca ctgctgccag tactttgtga
     1261 accaattatg gacgatttgc cagtagagac tatcaccaag cagctccaaa ttagacggct
     1321 agaaaaacaa attggcaaca aagatgttaa gaattccgga tccactgtgc ttagtgacga
     1381 tttctcaaca tctcaagagg gagcgatatc ggaggatact aacaaacctc ctgaactgaa
     1441 aagtaaaaat caattgtggc aagagctaaa aatcaaagca ataacaagat ttctcacgct
     1501 gatatattgt gaatccttgc tgattgtatt cttacacctt cagcttaata ttctatcgcg
     1561 caagtcttat cttgagactg caatcaggct ggcttctgag acccagggga tagatttggt
     1621 agatcaagag tctaatggtg acttttctgg caacactcag gatgaaaact tatccgaaca
     1681 agcattcttg agttttagtt ggtggctgct caacaaggga tggcttgaga taaagaacaa
     1741 gattgagcca tgtgttgaac aacactttgg aggtataaat cccagacagc aattgaagat
     1801 aaatgagttt gccgagcttt taaacaaatg tcaaaattgt attgacctga aggttttaaa
     1861 tcttaccgaa gaggatatac atttgggagt cggtgttatc gaagaccagt cccagcctgt
     1921 tggaagaaaa agcactaact ttatcacaaa cgctttatta ccacccaaag agtttgagtt
     1981 tttccttttg caacagacaa atgatttaga ctttttatcc cgcttcaaca ataatatcgt
     2041 caatactgag agcttgaaca tgttattgga tgaattaaac aactatctga acaatgcgga
     2101 catcaatctg atagtcaaca aattggctac cctaggtatt accaaagttc tggatgaaat
     2161 agttctcaac ctattacaga agaatcgacc gaacccaatt tcagatatga atatacgtga
     2221 tattgatctc aatgattttc ccccctacaa attagcagct ttgctggcaa acataaccaa
     2281 acagtctatt tcgttgacta acaactctgt ggagaaccct attttagcag acttaaacaa
     2341 tttaccggaa cttaatgatc tcagcgcaag tgtatactct aattttgatc ctcgctcgag
     2401 atccatgggt ggtggcggcc gcggtagaac ttctcaaaaa ccgttgatgg agcgtgtttc
     2461 tgtcgacccg ccgttcaccg tgtctgacct gaaacaagct atcccgccac actgtttcaa
     2521 gcgttctgtg atcagatctt cttactacat cgtacacgac gcgataatcg cttacatttt
     2581 ctacttcctg gctgacaaat acatccctat tctgccagcc ccgctagcct acctagcttg
     2641 gccgctgtat tggttctgtc aagcttctat cctgaccggt ctgtgggtaa tcggccacga
     2701 gtgcggtcac cacgcgttct ctgactacca gtgggtagac gacaccgttg gtttcatttt
     2761 gcactctttc ctaatgactc catacttcag ctggaaatac tctcaccgta accaccacgc
     2821 taacacgaat tctctggaca acgatgaggt ttacatcccg aaatctaaag ccaaagtagc
     2881 tctgtactat aaagttctga accacccccc gggtcgttta ttaatcatgt tcatcacctt
     2941 cactttaggt ttcccattat acctcttcac caacatctct ggtaaaaaat acgagcgttt
     3001 cgctaaccac ttcgatccga tgagtccaat cttcaaggag agagagagat tccaggttct
     3061 gctgtccgac ctaggtctac tagctgtttt atacggtgtt aaattggctg ttgctgctaa
     3121 gggtgccgct tgggtaacct gcatctatgg gatcccggtt ctaggcgtgt tcatcttctt
     3181 cgatatcatc acttacctgc accacaccca cctgtccttg ccacactacg actcttctga
     3241 gtggaactgg ctgcgtggag ctctatctac catcgacaga gacttcggtt tcctgaactc
     3301 tgttctgcac gatgttaccc acacccacgt aatgcatcac ttgttcagct acatcccgca
     3361 ctaccacgct aaggaggcta gagacgctat caacactgtt ctgggcgact tctacaaaat
     3421 cgatagaact ccaatattga aagccatgtg gcgtgaggct aaggagtgca tcttcatcga
     3481 gccagagaaa ggcagagagt ctaaaggtgt ttactggtac aacaaattcg cggggcccga
     3541 acaaaaactc atctcagaag aggatctgaa tagcgccgtc gaccatcatc atcatcatca
     3601 ttgagtttta gccttagaca tgactgttcc tcagttcaag ttgggcactt acgagaagac
     3661 cggtcttgct agattctaat caagaggatg tcagaatgcc atttgcctga gagatgcagg
     3721 cttcattttt gatacttttt tatttgtaac ctatatagta taggattttt tttgtcattt
     3781 tgtttcttct cgtacgagct tgctcctgat cagcctatct cgcagctgat gaatatcttg
     3841 tggtaggggt ttgggaaaat cattcgagtt tgatgttttt cttggtattt cccactcctc
     3901 ttcagagtac agaagattaa gtgagacctt cgtttgtgcg gatcccccac acaccatagc
     3961 ttcaaaatgt ttctactcct tttttactct tccagatttt ctcggactcc gcgcatcgcc
     4021 gtaccacttc aaaacaccca agcacagcat actaaatttt ccctctttct tcctctaggg
     4081 tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa agagaccgcc tcgtttcttt
     4141 ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt ctttttcttg aaattttttt
     4201 ttttagtttt tttctctttc agtgacctcc attgatattt aagttaataa acggtcttca
     4261 atttctcaag tttcagtttc atttttcttg ttctattaca acttttttta cttcttgttc
     4321 attagaaaga aagcatagca atctaatcta aggggcggtg ttgacaatta atcatcggca
     4381 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     4441 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     4501 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     4561 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     4621 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     4681 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     4741 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     4801 cacgtccgac ggcggcccac gggtcccagg cctcggagat ccgtccccct tttcctttgt
     4861 cgatatcatg taattagtta tgtcacgctt acattcacgc cctcccccca catccgctct
     4921 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt
     4981 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg
     5041 cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa
     5101 ggctttaatt tgcaagctgg agaccaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5161 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5221 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5281 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5341 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     5401 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5461 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5521 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5581 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     5641 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5701 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5761 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5821 cgaaaactca cgttaaggga ttttggtcat gagatc
//
