LOCUS       sequence-140438-         519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpCas9, GFP, and sgRNA targeting Ago1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Ngondo RP, Cirera-Salinas D, Yu J, Wischnewski H, Bodak M, 
            Vandormael-Pournin S, Geiselmann A, Wettstein R, Luitz J, 
            Cohen-Tannoudji M, Ciaudo C
  TITLE     Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation
            of Mouse Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023.
  PUBMED    29396181
REFERENCE   2  (bases 1 to 519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023."
COMMENT     Sequence Label: pX458-sgRNA_Ago1_4
FEATURES             Location/Qualifiers
     source          1..519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(299..319)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(326..391)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      391..400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccttggccat ctcgttgctg aagatctctt gcagatagca gatccggttc ttccgtctgg
       61 tgtatcttct tctggcggtt ctcttcagcc gggtggcctc ggctgtttcg ccgctgtcga
      121 acagcagggc tccgatcagg ttcttcttga tgctgtgccg gtcggtgttg cccagcacct
      181 tgaatttctt gctgggcacc ttgtactcgt cggtgatcac ggcccagccc acagagttgg
      241 tgccgatgtc caggccgatg ctgtacttct tgtcggctgc tgggactccg tggataccga
      301 ccttccgctt cttctttggg gccatcttat cgtcatcgtc tttgtaatca atatcatgat
      361 ccttgtagtc tccgtcgtgg tccttatagt ccatggtggc accggtccaa cctgaaaaaa
      421 agtgatttca ggcaggtgct ccaggtaatt aaacattaat accccaccaa ccaaccatcc
      481 cttaaaccct tacctcttgc tcagctaatt acagcccgg
//
