LOCUS       sequence-140461-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with 
            N-terminal His6 tag (H6YfdE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Mullins EA, Sullivan KL, Kappock TJ
  TITLE     Function and X-ray crystal structure of Escherichia coli YfdE.
  JOURNAL   PLoS One. 2013 Jul 23;8(7):e67901. doi: 
            10.1371/journal.pone.0067901. Print 2013.
  PUBMED    23935849
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-23"; volume: "8(7):e67901. doi"; pages: " 
            10.1371/journal.pone.0067901. Print 2013"
COMMENT     Sequence Label: pJK593
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgac aaataatgaa agcaaagggc
      121 cgtttgaagg cttattagtt atcgatatga cacatgtcct taatggacct ttcggaactc
      181 aacttctttg taatatgggc gcaagggtaa ttaaagttga gccgccgggt catggtgatg
      241 atacccgcac atttggtccc tatgtggatg gacagtcact ctattacagt tttattaatc
      301 atggcaaaga gagtgtggtt cttgatttaa agaatgatca cgataaaagt atatttataa
      361 atatgctcaa acaagctgat gtattagctg agaattttcg cccaggtaca atggaaaaac
      421 tggggttttc atgggaaacg cttcaagaaa tcaacccgcg cctcatatat gcttcatcgt
      481 caggtttcgg acataccggt ccgctaaaag atgctcctgc ctacgatacc atcattcagg
      541 caatgagcgg gataatgatg gaaacaggat atcctgatgc tccgccagtg cgcgttggta
      601 catctcttgc ggatctatgc ggcggtgtct atttattcag cggaatagtg agtgcacttt
      661 atggtcgcga aaagagccag agaggggcgc atgtcgatat agcgatgttt gatgccacgc
      721 tgagttttct ggagcatggt ctgatggcat atatcgcaac tgggaagtca ccacaacgtc
      781 tgggaaatcg ccatccctac atggcacctt ttgatgtttt caatactcag gataagccga
      841 ttacgatttg ttgtggtaat gacaagcttt tttctgcgtt atgccaggca ctggagctta
      901 cggaactggt taatgatccc cgatttagca gcaatatttt acgcgtacaa aaccaggcta
      961 ttcttaaaca atatattgag cggacgttaa aaacgcaggc agctgaagtt tggttagcca
     1021 gaatacatga agttggtgta cccgtcgcgc cgttattaag tgtggctgag gccattaaat
     1081 tgcc
//
