LOCUS       sequence-140462-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with 
            N-terminal His6 tag (H6YfdE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Mullins EA, Sullivan KL, Kappock TJ
  TITLE     Function and X-ray crystal structure of Escherichia coli YfdE.
  JOURNAL   PLoS One. 2013 Jul 23;8(7):e67901. doi: 
            10.1371/journal.pone.0067901. Print 2013.
  PUBMED    23935849
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-23"; volume: "8(7):e67901. doi"; pages: " 
            10.1371/journal.pone.0067901. Print 2013"
COMMENT     Sequence Label: pJK593
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt tatgatgaga
       61 actcctggcg aatttgttcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg
      121 ggtccgcgca gccgctgatt tttatcggat tacccggcat cattattccc ccggcttcaa
      181 tcaacatatt tctcgcctga gtttgtggca atttaatggc ctcagccaca cttaataacg
      241 gcgcgacggg tacaccaact tcatgtattc tggctaacca aacttcagct gcctgcgttt
      301 ttaacgtccg ctcaatatat tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc
      361 tgctaaatcg gggatcatta accagttccg taagctccag tgcctggcat aacgcagaaa
      421 aaagcttgtc attaccacaa caaatcgtaa tcggcttatc ctgagtattg aaaacatcaa
      481 aaggtgccat gtagggatgg cgatttccca gacgttgtgg tgacttccca gttgcgatat
      541 atgccatcag accatgctcc agaaaactca gcgtggcatc aaacatcgct atatcgacat
      601 gcgcccctct ctggctcttt tcgcgaccat aaagtgcact cactattccg ctgaataaat
      661 agacaccgcc gcatagatcc gcaagagatg taccaacgcg cactggcgga gcatcaggat
      721 atcctgtttc catcattatc ccgctcattg cctgaatgat ggtatcgtag gcaggagcat
      781 cttttagcgg accggtatgt ccgaaacctg acgatgaagc atatatgagg cgcgggttga
      841 tttcttgaag cgtttcccat gaaaacccca gtttttccat tgtacctggg cgaaaattct
      901 cagctaatac atcagcttgt ttgagcatat ttataaatat acttttatcg tgatcattct
      961 ttaaatcaag aaccacactc tctttgccat gattaataaa actgtaatag agtgactgtc
     1021 catccacata gggaccaaat gtgcgggtat catcaccatg acccggcggc tcaactttaa
     1081 ttacccttgc gcccata
//
