LOCUS       sequence-140468-       10197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Macpherson LJ, Zaharieva EE, Kearney PJ, Alpert MH, Lin TY, Turan Z,
            Lee CH, Gallio M
  TITLE     Dynamic labelling of neural connections in multiple colours by 
            trans-synaptic fluorescence complementation.
  JOURNAL   Nat Commun. 2015 Dec 4;6:10024. doi: 10.1038/ncomms10024.
  PUBMED    26635273
REFERENCE   2  (bases 1 to 10197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-12-4"; volume: "6:10024. doi"; pages: " 
            10.1038/ncomms10024"
COMMENT     Sequence Label: pLOT-syb-sp-YFP1-10
FEATURES             Location/Qualifiers
     source          1..10197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3224..3462
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3409..3430
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             4101..4742
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQTVLSKDPNEK"
     intron          4835..4900
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5030..5050
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5322..5456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5359..5378)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5413..5432
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     gene            5474..9601
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(5483..5501)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5952..6023,6418..6691,6766..7420,7482..7797,8018..8149,
                     8220..8834)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9612..10166,10167..10197)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atcctaatct tacctcgact gctgtatata
     3061 aaaccagtgg ttatatgtac agtactgctg tatataaaac cagtggttat atgtacagta
     3121 cgtcgactgc tgtatataaa accagtggtt atatgtacag tactgctgta tataaaacca
     3181 gtggttatat gtacagtacg tcgagggatg ataagcttga gcgccggagt ataaatagag
     3241 gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc
     3301 gaaagctaag caaataaaca agcgcagctg agcaagctaa acaatctgca gtaaagtgca
     3361 agttaaagtg aatcaattaa aagtaaccag caaccaagta aatcaactgc aactactgaa
     3421 atctgccaag aagtaattat tgaatacaag gagagaactc tgaataggga attgggaatt
     3481 cgctgaggcc accagagcac caccagccaa accacagaag caaccacaca gccaccagag
     3541 ccatggcgga cgctgcacca gctggcgatg caccacccaa tgccggagcc ccggccggag
     3601 agggcggcga tggcgagatt gtgggcggac cacacaatcc gcagcagatc gcggcacaga
     3661 agcgtctgca gcagacgcag gcgcaggtcg atgaggtcgt ggacatcatg cgcacgaacg
     3721 tggagaaggt gctggagcgc gacagcaagc tgtcggagct ggacgaccgt gccgatgcct
     3781 tgcagcaggg tgcctcgcag tttgagcagc aggcgggcaa gctcaagagg aaattctggc
     3841 ttcagaactt aaagatgatg atcatcatgg gcgtgattgg cctggttgtc gtgggcatta
     3901 ttgcaaataa acttggactc ataggcgggg agcagccgcc acagtaccag tatcccccac
     3961 agtacatgca gccaccgccg ccgccaccgc agcagccagc cggaggacag tcatcgctgg
     4021 tggatgcggc aggagcagga gacggagcag gagcaggagg atcggcagga gctggcgatc
     4081 acggcggcgt ggctcgagga atgtccaaag gagaagaact gtttaccggt gttgtgccaa
     4141 ttttggttga actcgatggt gatgtcaacg gacataagtt ctcagtgaga ggcgaaggag
     4201 aaggtgacgc caccattgga aaattgactc ttaaattcat ctgtactact ggtaaacttc
     4261 ctgtaccatg gccgactctc gtaacaacgc ttgggtacgg acttcagtgc tttgcgagat
     4321 acccagacca tatgaaaaga catgactttt ttaagtcggc tatgcctgaa ggttacgtgc
     4381 aagagagaac aatttcgttc aaagatgatg gaaaatataa aactagagca gttgttaaat
     4441 ttgaaggaga tactttggtt aaccgcattg aactgaaagg aacagatttt aaagaagatg
     4501 gtaatattct tggacacaaa ctcgaataca attttaatag tcataacgta tacatcactg
     4561 ctgataagca aaagaacgga attaaagcga atttcacagt acgccataat gtagaagatg
     4621 gcagtgttca acttgccgac cattaccaac aaaacacccc tattggagac ggtccggtac
     4681 ttcttcctga taatcactac ctctcatacc aaacagtcct gagcaaagat ccaaatgaaa
     4741 aaggaacata gtctagagga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     4801 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaagtttt taagtgtata
     4861 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     4921 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     4981 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     5041 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     5101 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     5161 actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga ctagagatca
     5221 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     5281 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     5341 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5401 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccact
     5461 agaaggccta attctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat
     5521 aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct gatcagtttt
     5581 aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac ctctcactca
     5641 aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg
     5701 ccgtctcttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacgtagc
     5761 gtcaatgtcc gccttcagtt gcttttgtca gcggtttcgt gacgaagctc caagcggttt
     5821 acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt atttttgttt
     5881 gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg aaagtgtgaa
     5941 aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc agcaaacacc
     6001 catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta agttcacttg
     6061 atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca
     6121 aaaatcccaa accgcaatcg cacaaacaaa tagtgacacg aaacagatta ttctggtagc
     6181 tgtgctcgct atataagaca atttttaaga tcatatcatg atcaagacat ctaaaggcat
     6241 tcattttcga ctacattctt ttttacaaaa aatataacaa ccagatattt taagctgatc
     6301 ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact tcgttttgtt
     6361 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     6421 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     6481 ggcacgctcc tgccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     6541 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     6601 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     6661 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     6721 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     6781 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     6841 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     6901 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     6961 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     7021 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     7081 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     7141 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     7201 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     7261 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     7321 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     7381 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     7441 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     7501 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     7561 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     7621 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     7681 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     7741 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     7801 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     7861 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     7921 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     7981 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     8041 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     8101 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     8161 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     8221 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     8281 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     8341 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     8401 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     8461 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     8521 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     8581 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     8641 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     8701 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     8761 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     8821 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt ttaccattat
     8881 taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca
     8941 ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt
     9001 agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa
     9061 ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg
     9121 cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc
     9181 tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt
     9241 tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg tctcaataga
     9301 aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg tatatatttc
     9361 cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat tttttgtgac
     9421 ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg tttgttcctt
     9481 gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata tatcaaaaat
     9541 aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc actggaacta
     9601 gaattaattc ggctgctgct ctaaacgacg catttcgtac tccaaagtac gaattttttc
     9661 cctcaagctc ttattttcat taaacaatga acaggaccta acgcacagtc acgttattgt
     9721 ttacataaat gatttttttt actattcaaa cttactctgt ttgtgtactc ccactggtat
     9781 agccttcttt tatcttttct ggttcaggct ctatcacttt actaggtacg gcatctgcgt
     9841 tgagtcgcct ccttttaaat gtctgacctt ttgcaggtgc agccttccac tgcgaatcat
     9901 taaagtgggt atcacaaatt tgggagtttt caccaaggct gcacccaagg ctctgctccc
     9961 acaattttct cttaatagca cacttcggca cgtgaattaa ttttactcca gtcacagctt
    10021 tgcagcaaaa tttgcaatat ttcatttttt tttattccac gtaagggtta atgttttcaa
    10081 aaaaaaattc gtccgcacac aacctttcct ctcaacaagc aaacgtgcac tgaatttaag
    10141 tgtatacttc ggtaagcttc ggctatcgac gggaccacct tatgttattt catcatg
//
