LOCUS       sequence-140469-       10303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Macpherson LJ, Zaharieva EE, Kearney PJ, Alpert MH, Lin TY, Turan Z,
            Lee CH, Gallio M
  TITLE     Dynamic labelling of neural connections in multiple colours by 
            trans-synaptic fluorescence complementation.
  JOURNAL   Nat Commun. 2015 Dec 4;6:10024. doi: 10.1038/ncomms10024.
  PUBMED    26635273
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-12-4"; volume: "6:10024. doi"; pages: " 
            10.1038/ncomms10024"
COMMENT     Sequence Label: pUAS-syb:spGFP1–10
FEATURES             Location/Qualifiers
     source          1..10303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    135..229
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        248..486
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     433..454
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             1125..1766
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     intron          1859..1924
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2054..2074
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2499..2633
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2536..2555)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2590..2609
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2660..2678)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3138..3209,3604..3877,3952..4606,4668..4983,5204..5335,
                     5406..6020)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(6798..7352,7353..7383)
                     /label=P element 5' end
     rep_origin      complement(7618..8206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7698..7717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8377..9168,9169..9237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9000..9019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9238..9342)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9410..9428
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9466..9488)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9588..9607
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(10144..10174,10175..10303,1..73)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 ttgaattaac tcataatatt aattagacga aattattttt aaagttttat ttttaataat
       61 ttgcgagtac gcaaagcttg gctgcatcca acgcgttggg agctctccgg atccaagctt
      121 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
      181 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
      241 gcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca
      301 agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag
      361 ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca
      421 agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga
      481 actctgaata gggaattggg aattcgctga ggccaccaga gcaccaccag ccaaaccaca
      541 gaagcaacca cacagccacc agagccatgg cggacgctgc accagctggc gatgcaccac
      601 ccaatgccgg agccccggcc ggagagggcg gcgatggcga gattgtgggc ggaccacaca
      661 atccgcagca gatcgcggca cagaagcgtc tgcagcagac gcaggcgcag gtcgatgagg
      721 tcgtggacat catgcgcacg aacgtggaga aggtgctgga gcgcgacagc aagctgtcgg
      781 agctggacga ccgtgccgat gccttgcagc agggtgcctc gcagtttgag cagcaggcgg
      841 gcaagctcaa gaggaaattc tggcttcaga acttaaagat gatgatcatc atgggcgtga
      901 ttggcctggt tgtcgtgggc attattgcaa ataaacttgg actcataggc ggggagcagc
      961 cgccacagta ccagtatccc ccacagtaca tgcagccacc gccgccgcca ccgcagcagc
     1021 cagccggagg acagtcatcg ctggtggatg cggcaggagc aggagacgga gcaggagcag
     1081 gaggatcggc aggagctggc gatcacggcg gcgtggctcg aggaatgtcc aaaggagaag
     1141 aactgtttac cggtgttgtg ccaattttgg ttgaactcga tggtgatgtc aacggacata
     1201 agttctcagt gagaggcgaa ggagaaggtg acgccaccat tggaaaattg actcttaaat
     1261 tcatctgtac tactggtaaa cttcctgtac catggccgac tctcgtaaca acgcttacgt
     1321 acggagttca gtgcttttcg agatacccag accatatgaa aagacatgac ttttttaagt
     1381 cggctatgcc tgaaggttac gtgcaagaaa gaacaatttc gttcaaagat gatggaaaat
     1441 ataaaactag agcagttgtt aaatttgaag gagatacttt ggttaaccgc attgaactga
     1501 aaggaacaga ttttaaagaa gatggtaata ttcttggaca caaactcgaa tacaatttta
     1561 atagtcataa cgtatacatc actgctgata agcaaaagaa cggaattaaa gcgaatttca
     1621 cagtacgcca taatgtagaa gatggcagtg ttcaacttgc cgaccattac caacaaaaca
     1681 cccctattgg agacggtccg gtacttcttc ctgataatca ctacctctca acacaaacag
     1741 tcctgagcaa agatccaaat gaaaaaggaa catagtctag aggatctttg tgaaggaacc
     1801 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     1861 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     1921 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     1981 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     2041 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     2101 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     2161 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     2221 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     2281 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     2341 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     2401 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2461 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     2521 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2581 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcggatcca
     2641 ctagaaggcc ttagtatgta tgtaagttaa taaaaccctt ttttggagaa tgtagattta
     2701 aaaaaacata tttttttttt attttttact gcactggaca tcattgaact tatctgatca
     2761 gttttaaatt tacttcgatc caagggtatt tgaagtacca ggttctttcg attacctctc
     2821 actcaaaatg acattccact caaagtcagc gctgtttgcc tccttctctg tccacagaaa
     2881 tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc gccaccgttt gtagcgttac
     2941 ctagcgtcaa tgtccgcctt cagttgcact ttgtcagcgg tttcgtgacg aagctccaag
     3001 cggtttacgc catcaattaa acacaaagtg ctgtgccaaa actcctctcg cttcttattt
     3061 ttgtttgttt tttgagtgat tggggtggtg attggttttg ggtgggtaag caggggaaag
     3121 tgtgaaaaat cccggcaatg ggccaagagg atcaggagct attaattcgc ggaggcagca
     3181 aacacccatc tgccgagcat ctgaacaatg tgagtagtac atgtgcatac atcttaagtt
     3241 cacttgatct ataggaactg cgattgcaac atcaaattgt ctgcggcgtg agaactgcga
     3301 cccacaaaaa tcccaaaccg caatcgcaca aacaaatagt gacacgaaac agattattct
     3361 ggtagctgtg ctcgctatat aagacaattt ttaagatcat atcatgatca agacatctaa
     3421 aggcattcat tttcgactac attctttttt acaaaaaata taacaaccag atattttaag
     3481 ctgatcctag atgcacaaaa aataaataaa agtataaacc tacttcgtag gatacttcgt
     3541 tttgttcggg gttagatgag cataacgctt gtagttgata tttgagatcc cctatcattg
     3601 cagggtgaca gcggagcggc ttcgcagagc tgcattaacc agggcttcgg gcaggccaaa
     3661 aactacggca cgctcctgcc acccagtccg ccggaggact ccggttcagg gagcggccaa
     3721 ctagccgaga acctcaccta tgcctggcac aatatggaca tctttggggc ggtcaatcag
     3781 ccgggctccg gatggcggca gctggtcaac cggacacgcg gactattctg caacgagcga
     3841 cacataccgg cgcccaggaa acatttgctc aagaacggtg agtttctatt cgcagtcggc
     3901 tgatctgtgt gaaatcttaa taaagggtcc aattaccaat ttgaaactca gtttgcggcg
     3961 tggcctatcc gggcgaactt ttggccgtga tgggcagttc cggtgccgga aagacgaccc
     4021 tgctgaatgc ccttgccttt cgatcgccgc agggcatcca agtatcgcca tccgggatgc
     4081 gactgctcaa tggccaacct gtggacgcca aggagatgca ggccaggtgc gcctatgtcc
     4141 agcaggatga cctctttatc ggctccctaa cggccaggga acacctgatt ttccaggcca
     4201 tggtgcggat gccacgacat ctgacctatc ggcagcgagt ggcccgcgtg gatcaggtga
     4261 tccaggagct ttcgctcagc aaatgtcagc acacgatcat cggtgtgccc ggcagggtga
     4321 aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc ctccgaggca ctaaccgatc
     4381 cgccgcttct gatctgcgat gagcccacct ccggactgga ctcatttacc gcccacagcg
     4441 tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac cgtcatcctg accattcatc
     4501 agccgtcttc cgagctgttt gagctctttg acaagatcct tctgatggcc gagggcaggg
     4561 tagctttctt gggcactccc agcgaagccg tcgacttctt ttcctagtga gttcgatgtg
     4621 tttattaagg gtatctagca ttacattaca tctcaactcc tatccagcgt gggtgcccag
     4681 tgtcctacca actacaatcc ggcggacttt tacgtacagg tgttggccgt tgtgcccgga
     4741 cgggagatcg agtcccgtga tcggatcgcc aagatatgcg acaattttgc tattagcaaa
     4801 gtagcccggg atatggagca gttgttggcc accaaaaatt tggagaagcc actggagcag
     4861 ccggagaatg ggtacaccta caaggccacc tggttcatgc agttccgggc ggtcctgtgg
     4921 cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa aagtgcgact tattcagaca
     4981 acggtgagtg gttccagtgg aaacaaatga tataacgctt acaattcttg gaaacaaatt
     5041 cgctagattt tagttagaat tgcctgattc cacacccttc ttagtttttt tcaatgagat
     5101 gtatagttta tagttttgca gaaaataaat aaatttcatt taactcgcga acatgttgaa
     5161 gatatgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg ccatcttgat
     5221 tggcctcatc tttttgggcc aacaactcac gcaagtgggc gtgatgaata tcaacggagc
     5281 catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga taaatgtaag
     5341 tcttgtttag aatacatttg catattaata atttactaac tttctaatga atcgattcga
     5401 tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt cgactttatc
     5461 gctgtgacac atactttctg ggcaaaacga ttgccgaatt accgcttttt ctcacagtgc
     5521 cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga gtgctgcact
     5581 tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc
     5641 taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg ccggttatca
     5701 taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca gtatacctca
     5761 aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg attaaccaat
     5821 gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg tgccccagtt
     5881 cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg ctggactacg
     5941 tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct ctaagacttc
     6001 gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt ttttttttac
     6061 cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt
     6121 gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc
     6181 acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat
     6241 caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata catacttttc
     6301 aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc
     6361 gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac
     6421 tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc atttcgtctc
     6481 aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata tatttgtata
     6541 tatttccctc acagctatat ttattctaat ttaatattat gactttttaa ggtaattttt
     6601 tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat ccagtgtttg
     6661 ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt tatatatatc
     6721 aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg gatttcactg
     6781 gaactagaat taattcggct gctgctctaa acgacgcatt tcgtactcca aagtacgaat
     6841 tttttccctc aagctcttat tttcattaaa caatgaacag gacctaacgc acagtcacgt
     6901 tattgtttac ataaatgatt ttttttacta ttcaaactta ctctgtttgt gtactcccac
     6961 tggtatagcc ttcttttatc ttttctggtt caggctctat cactttacta ggtacggcat
     7021 ctgcgttgag tcgcctcctt ttaaatgtct gaccttttgc aggtgcagcc ttccactgcg
     7081 aatcattaaa gtgggtatca caaatttggg agttttcacc aaggctgcac ccaaggctct
     7141 gctcccacaa ttttctctta atagcacact tcggcacgtg aattaatttt actccagtca
     7201 cagctttgca gcaaaatttg caatatttca ttttttttta ttccacgtaa gggttaatgt
     7261 tttcaaaaaa aaattcgtcc gcacacaacc tttcctctca acaagcaaac gtgcactgaa
     7321 tttaagtgta tacttcggta agcttcggct atcgacggga ccaccttatg ttatttcatc
     7381 atgggccaga cccacgtagt ccagcggcag atcggcggcg gagaagttaa gcgtctccag
     7441 gatgaccttg cccgaactgg ggcacgtggt gttcgacgat gtgcagctaa tttcgcccgg
     7501 ctccacgtcc gcccattggt taatcagcag accctcgttg gcgtaacgga accatgagag
     7561 gtacgacaac catttgaggt atactggcac cgagcccgag ttcaagaaga aggcgttttt
     7621 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7681 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7741 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7801 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7861 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7921 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7981 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8041 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8101 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8161 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8221 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8281 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8341 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8401 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8461 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8521 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8581 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8641 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8701 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8761 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8821 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8881 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8941 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     9001 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9061 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9121 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9181 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9241 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9301 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9361 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     9421 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     9481 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     9541 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     9601 gattgtactg agagtgcacc atatgcggtg tgaaataccg caccgaatcg cgcggaacta
     9661 acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga
     9721 gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga agagggcgcg
     9781 cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc gataaacgag
     9841 tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct
     9901 acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc ttgtttttat
     9961 tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa cacgatggta
    10021 ataatggtaa aaaaaaaaac aagcagttat ttcggatata tgtcggctac tccttgcgtc
    10081 gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg agaatggcca
    10141 gaccatgatg aaataacata aggtggtccc gtcggcaaga gacatccact taacgtatgc
    10201 ttgcaataag tgcgagtgaa aggaatagta ttctgagtgt cgtattgagt ctgagtgaga
    10261 cagcgatatg attgttgatt aacccttagc atgtccgtgg ggt
//
