LOCUS       sequence-140470-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in E.coli B. bacteriovorus Bd3179.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   de la Pena AH, Suarez A, Duong-Ly KC, Schoeffield AJ, Pizarro-Dupuy 
            MA, Zarr M, Pineiro SA, Amzel LM, Gabelli SB
  TITLE     Structural and Enzymatic Characterization of a Nucleoside 
            Diphosphate Sugar Hydrolase from Bdellovibrio bacteriovorus.
  JOURNAL   PLoS One. 2015 Nov 2;10(11):e0141716. doi: 
            10.1371/journal.pone.0141716. eCollection 2015.
  PUBMED    26524597
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-11-2"; volume: "10(11):e0141716. doi"; pages: " 
            10.1371/journal.pone.0141716. eCollection 2015"
COMMENT     Sequence Label: pET24_BD3179
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             635..657
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    672..696
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(696..715)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(697..715)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(799..818)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatcctc aattccactc ccctcttaga aatttatcaa gccaaaaagc
      121 cccgatctgg gttttcacat cgctcacttt tccctcgaga acaagctgca taaggtctgc
      181 gggttttact tcgacaacct caataaactc accctgatcc agacgctgct ccagatgagt
      241 caggtccctg gccagataca aatcaatgtg ctcgttggaa tagccaatga caggatggat
      301 tgtggtcagg aacttccagt ctttggcttc atagccggtc tcttcgagca gctctctttt
      361 tgccgtcaga agggtttctt cgttgtggtc tcgttttccg gcaggaaact cgagaaaaac
      421 ttttttaacc gcgtgacggt actgatgaat catcaccaca ttcccatttg gcaaaagcgg
      481 aatcatcatc gcggcgccgg gatgcagaat gtactcacgg gtgtaggtgc gcccgtcggg
      541 agcctgcacc tgatcctgtt caattttgag gtatcgtcct ttaaaaatct gacgcgtgga
      601 cagcgtcttt tcttccaaat gcttcatatg tatatctcct tcttaaagtt aaacaaaatt
      661 atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc
      721 gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
      781 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tgg
//
