LOCUS       sequence-140471-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant 
            E113A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Duong-Ly KC, Woo HN, Dunn CA, Xu W, Babic A, Bessman MJ, Amzel LM, 
            Gabelli SB
  TITLE     A UDP-X diphosphatase from Streptococcus pneumoniae hydrolyzes 
            precursors of peptidoglycan biosynthesis.
  JOURNAL   PLoS One. 2013 May 15;8(5):e64241. doi: 
            10.1371/journal.pone.0064241. Print 2013.
  PUBMED    23691178
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-15"; volume: "8(5):e64241. doi"; pages: " 
            10.1371/journal.pone.0064241. Print 2013"
COMMENT     Sequence Label: pET24a_UDP_Xase_E113
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             702..724
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagcttgtc
       61 gacggagctc gaattcggat ccttagtcaa gatattgccc cctatgacct tgataaacct
      121 gccaaagaag ctctatttgc tccttggtaa tgcgtttttc agataagttc ggcagttggt
      181 caatggcaaa aaattgaagg tcagcaattt cttgattttc ttggaattgt ccatcaagaa
      241 gcttacatcc aaaaacaaac tttgtatact gtttgctctg tagttggaaa cgattggtat
      301 caaaaacagc tagcagtctt tcaactttgg ctttaaaacc ggtttcttct tcaattgcct
      361 tgagaatatt ttctgttgga gaatagccga cttcaccaaa gccacctggc aaagcccaac
      421 tatcctctcc ttgtccccta accagacaaa tcttctcatc ctcaacaatc caagcacgga
      481 cgtccattaa cggagtcgca taagcagaag ttggcttcaa gacttctgcc acttcttcgg
      541 aatcaaggtc tgatgcttga ttcaacattt cagataacag acttcgcaag tcttcgtagc
      601 gctcacggtc aaacggatct tttgtaaagg ttaagccagt atctgtaatg gcaatcattc
      661 tttgcaaata cttgacaaaa tcacttgtct tcatatgtat atctccttct taaagttaaa
      721 caaaattatt tctagaggg
//
