LOCUS       sequence-140472-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant 
            E162A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Duong-Ly KC, Woo HN, Dunn CA, Xu W, Babic A, Bessman MJ, Amzel LM, 
            Gabelli SB
  TITLE     A UDP-X diphosphatase from Streptococcus pneumoniae hydrolyzes 
            precursors of peptidoglycan biosynthesis.
  JOURNAL   PLoS One. 2013 May 15;8(5):e64241. doi: 
            10.1371/journal.pone.0064241. Print 2013.
  PUBMED    23691178
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-15"; volume: "8(5):e64241. doi"; pages: " 
            10.1371/journal.pone.0064241. Print 2013"
COMMENT     Sequence Label: pET24a_UDP_Xase_E162
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             698..720
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    735..759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(759..778)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(760..778)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatcctt agtcaagata ttgcccccta tgaccttgat aaacctgcca
      121 aagaagctct atttgctcct tggtaatgcg tttttcagat aagttcggca gttggtcaat
      181 ggcaaaaaat tgaaggtcag caattgcttg attttcttgg aattgtccat caagaagctt
      241 acatccaaaa acaaactttg tatactgttt gctctgtagt tggaaacgat tggtatcaaa
      301 aacagctagc agtctttcaa ctttggcttt aaaaccggtt tcttcttcaa tttccttgag
      361 aatattttct gttggagaat agccgacttc accaaagcca cctggcaaag cccaactatc
      421 ctctccttgt cccctaacca gacaaatctt ctcatcctca acaatccaag cacggacgtc
      481 cattaacgga gtcgcataag cagaagttgg cttcaagact tctgccactt cttcggaatc
      541 aaggtctgat gcttgattca acatttcaga taacagactt cgcaagtctt cgtagcgctc
      601 acggtcaaac ggatcttttg taaaggttaa gccagtatct gtaatggcaa tcattctttg
      661 caaatacttg acaaaatcac ttgtcttcat atgtatatct ccttcttaaa gttaaacaaa
      721 attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat
      781 ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc atcacc
//
